三種農用藥劑對小鼠精子活力、體外受精率及胚胎發育之影響

The Effects of Three Pesticides on Sperm Motility, in Vitro Fertilization and Embryo Development in Mice

呂水淵(Shui-Yuan Lu) ； 王順成(Shun-Cheng Wang)

《中華民國獸醫學會雜誌》 26卷1期 (2000/03) Pp. 24-35

https://doi.org/10.7009/JTCSVS.200003.0024

摘要

This study on reproductive toxicity was conducted utilizing teratogen, benomyl, nonteratogen, thiophanate-methyl in vivo test, and positive reproductive toxicity agent of Aroclor-1254 to investigate end point sperm motility, in vitro fertilization after 6-hour ovum-exposure was not influenced, to thiophanate-methyl while that to benomyl and Aroclor-1254 in midhigh doses was reduced (P＞0.05). In vitro fertilization after 2-hour sperm-exposure to thiophanate-methyl was comparable to that of the control while that to benomyl and Aroclor-1254 in high dose was decreased. Sperm motility showed no dose-response relationship though it was not comparable to that of the control during the 2-hour exposure. Sperm motility during the 8-and 10-hour exposure to the three chemicals were more significantly (P＜0.05) decreased than that of the control. Embryo development was influenced by the three chemicals in high doses during the 48-hour exposure while it was significantly (P＜0.05) reduced by those in low doses during the 96-hour exposure. To sum up, there were different degrees of influence on sperm motility, in vitro fertilization and embryo development. The effect of benomyl and Aroclor-1254 on these parameters were larger than that of thiophanate-methyl.

關鍵字

小鼠 ； 體外受精 ； 生殖毒性

並列摘要

This study on reproductive toxicity was conducted utilizing teratogen, benomyl, nonteratogen, thiophanate-methyl in vivo test, and positive reproductive toxicity agent of Aroclor-1254 to investigate end point sperm motility, in vitro fertilization after 6-hour ovum-exposure was not influenced, to thiophanate-methyl while that to benomyl and Aroclor-1254 in midhigh doses was reduced (P＞0.05). In vitro fertilization after 2-hour sperm-exposure to thiophanate-methyl was comparable to that of the control while that to benomyl and Aroclor-1254 in high dose was decreased. Sperm motility showed no dose-response relationship though it was not comparable to that of the control during the 2-hour exposure. Sperm motility during the 8-and 10-hour exposure to the three chemicals were more significantly (P＜0.05) decreased than that of the control. Embryo development was influenced by the three chemicals in high doses during the 48-hour exposure while it was significantly (P＜0.05) reduced by those in low doses during the 96-hour exposure. To sum up, there were different degrees of influence on sperm motility, in vitro fertilization and embryo development. The effect of benomyl and Aroclor-1254 on these parameters were larger than that of thiophanate-methyl.

並列關鍵字

Mouse ； In vitro fertilization ； Reproductive toxicity