- 期刊
Change of Potassium Current Density in Rabbit Corneal Epithelial Cells during Maturation and Cellular Senescence
並列摘要
Background: Voltage-gated potassium (K(superscript +)) channels may participate in cellular developmental regulation, including cell differentiation, proliferation and apoptosis. This study investigated the change of K(superscript +) current densities in corneal epithelial cells during maturation and cellular senescence. Methods: New Zealand white rabbits were divided into three age groups: newborn (≤ 7 days old, n=18); young (8-12 weeks old, n=59); and adult (20-28 weeks old, n=16). Rabbit corneal epithelial cells were subdivided into the following three groups: small cells with capacitance ＜6.0 pF; medium cells with capacitance 60-10.0 pF; and large cells with capacitance ＞10.0 pF. Using a whole-cell clamp technique, K(superscript +) current was recorded and current densities were calculated. Differences in K(superscript +) current densities among newborn, young and adult rabbits, as well as differences among small, medium and large cells, were analyzed. Results: We delineated two types of cells manifesting different amplitudes of depolarization-activated K(superscript +) outward currents. The averaged current density of type 1 response cells was significantly larger than that of type 2 cells in newborn, young, and adult groups. For newborn epithelial cells, the depolarization-gated outward K(superscript +) current density decreased from small to medium to large cells (p=0.049, at a membrane potential of 140 mV). A similar pattern of change in current density was also delineated for these cell sizes in young and adult rabbit corneal cells (p＜0.001 for both young and adult rabbits). An increase in depolarization-gated outward K(superscript +) current density was also delineated from newborn to young to adult rabbits (p＜0.001, p＜0.001 and p＜0.006 for small, medium and large cells, respectively, at a membrane potential of 140 mV). Conclusions: Corneal epithelial cells expressed K(superscript +) channel densities that were distinct from basal to superficial cells and from newborn to adult rabbits.
延伸閱讀
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