Distribution of Hypoxia-Inducible Factor-1α and Bcl-2-A Study of Varicose Veins by Confocal Microscopy

以共軛焦顯微鏡研究缺氧誘發因子－1α及Bcl-2在下肢靜脈曲張的分佈

易志成(Chin-Cheng Yi) ； 楊文凱(Wen-Kai Yang) ； 李建達(Jane-Dar Lee)

《Formosan Journal of Surgery》 43卷5期 (2010/10) Pp. 229-235

https://doi.org/10.29833/FJS.201010.0003

摘要

由於鬱血及靜脈壓力增加所導致的組織缺氧是下肢靜脈曲張的形成原因。減少細胞凋亡的Bcl-2（抗凋亡蛋白）可能對這些不健康的血管在膨脹跟管壁增厚上有所貢獻。在1公分片段的血管（肥厚型）來自12個因下肢大隱靜脈曲張而進行血管手術的病人。所有血管樣本利用免疫組織化學染色及共軛焦雷射掃描顯微鏡來研究缺氧誘發因子－1α（HIF-增厚）及Bcl-2在下肢靜脈曲張中的分佈與表現。
在下肢靜脈曲張中，HIF-1α在肥厚增粗的平滑肌層有較強的免疫染色反應。並且Bcl-2蛋白也是堆積在相同的平滑肌層。進一步以共軛焦雷射掃描顯微鏡研究獲得，大部分的HIF-1α分布在肌肉層，而Bcl-2蛋白在內皮層的表現比在肌肉層更高。
在共軛焦雷射掃描顯微鏡下，這些結果顯示大部分的HIF-1α及Bcl-2共定位在下肢靜脈曲張血管的肌肉層，且Bcl-2過度表現在血管的內皮層。

並列摘要

Objective: The tissue hypoxia caused by chronic venous insufficiency and blood stasis is the etiology of varicose vein formation. Bcl-2 (anti-apoptotic protein) reduces apoptosis may contribute to the dilated and thickened walls of the diseased vessels. We studied the distribution and relationship of hypoxia-inducible factor-1α (HIF-1α) and Bcl-2 in varicose veins by confocal microscopy.
Methods: This study consisted of examination of 1cm venous segments (hypertrophic type) from 12 patients obtained during conventional stripping surgery for the varicose saphenous vein in the calf muscle region. All vascular specimens were studied for HIF-1α and Bcl-2αby immunohistochemical stain and confocal laser scanning microscopy.
Results: In varicose veins, the immunohistochemical staining of both HIF-1αand Bcl-2 showed strong immunostaining in the hypertrophic smooth muscle layer. In further study, prominent HIF-1αdistribution in the muscle layer and Bcl-2 protein deposition in the endothelial layer were demonstrated under confocal laser scanning microscopy.
Conclusions: These results revealed prominent HIF-1αand Bcl-2 distribution in the muscle layer with co-localization, and Bcl-2 overexpression in the endothelial layer of varicose vein vessels under confocal microscopy.

並列關鍵字

HIF-1α ； Bcl-2 ； varicose vein ； confocal microscopy