Serological and Molecular Characterizations of a Hibiscus-infecting Tobamovirus in Taiwan

台灣地區感染朱槿的似菸草嵌紋病毒特性之分析

劉芳麟(Fang-Lin Liu) ； 陳宗祺(Tsung-Chi Chen) ； 葉錫東(Shyi-Dong Yeh) ； 徐惠迪(Hei-Ti Hsu) ； 陳慶忠(Ching-Chung Chen) ； 包慧俊(Huey-Jiunn Bau) ； 陳煜焜(Yuh-Kun Chen)

《植物病理學會刊》 13卷4期 (2004/12) Pp. 283-290

https://doi.org/10.6649/PPB.200412_13(4).0006

摘要

感染朱槿(Hibiscus rosa-sinensis L.)的菸嵌病毒屬病毒(Genus Tobamovirus)近來在國外陸續被報導，分析其寄主範圍、血清反應及鞘蛋白(coat protein，CP)和移動蛋白(movement protein，MP)基因的核苷酸序列等特性，顯示感染朱槿的桿狀病毒爲Tobamovirus屬的新種病毒。台灣地區也曾有朱槿受疑似菸嵌病毒感染之記綠。本研究針對感染台灣地區朱槿的菸嵌病毒之寄主範圍、血清親緣關係及鞘蛋白和移動蛋白等基因的核苷酸序列等特性進行進一步之分析。試驗結果顯示感染台灣地區朱槿的桿狀病毒與Tobamoviruses有血清類緣關係。在CP和MP的核苷酸序列及胺基酸序列上與Hibiscus latent Singapore virus(HLSV)有高達98%的相似度；而與Hibiscus latent Fort Pierce virus(HLFPV)則僅有71%的相似度。本研究之結果顯示台灣地區所分離之朱槿菸嵌病毒爲HLSV的一個分離株。

關鍵字

朱槿 ； 菸嵌病毒

並列摘要

A previously described hibiscus virus showing a tobamovirus profile was purified and used to produce polyclonal antisera in rabbit. The antiserum and viral RNA obtained in the study were serologically and molecularly characterized. The coat protein (CP) and movement protein (MP) genes of the virus were cloned and sequenced and the nucleic acid and the deduced amino acid sequences analyzed. The nucleic acid and amino acid sequences have high homology when compared with those of Hibiscus latent Singapore virus (HLSV, previously Hibiscus virus S, HVS) but less with those of Hibiscus latent Fort Pierce virus (HLFPV, previously Florida hibiscus virus, FHV). Results from current investigation indicate that the hibiscus virus cultures isolated in Taiwan are strains of HLSV.

並列關鍵字

hibiscus ； Hibiscus rosa-sinensis ； Tobamovirus