薑黃素微乳液之製備及其在超音波影響下對口腔鱗狀上皮細胞癌細胞株之抑制作用

Preparation of Curcumin Microemulsion and Their in vitro Suppression of Squamous Cell Carcinoma

李玫樺(Mei-Hwa Lee) ； 蔡斌智(Pin-Chin Tsai) ； 羅若玲(Jo-Lin Lo) ； 陳奐文(Huan-Wen Chen) ； 楊文仁(Wen-Jen Yang) ； 林宏殷(Hung-Yin Lin)

《台灣醫學》 16卷3期 (2012/05) Pp. 263-270

https://doi.org/10.6320/FJM.2012.16(3).06

摘要

近來越來越多很多使用天然物作為抗癌藥物，因為天然物有特性助於防止細胞損傷，可增加對癌症的抗氧化性。在此項研究中，以大豆卵磷脂、大豆油、聚山梨醇酯-80（Tween80）為組成，製備含薑黃素之微乳液，其成分都具有生物相容性，減少副作用。此薑黃素微乳液冷藏在5℃的薑黃素微乳液，至少可以穩定40天以上，其平均粒徑和表面電位（zeta potential）分別為46nm和-38mV。以薑黃素為細胞毒性劑，並以超音波促進藥物釋放是具有吸引力的。並在未施以超音波下，改變薑黃素微乳液的濃度並以口服攝入，對健康組織僅造成微小的影響。更重要的是超音波對體外藥物控制釋放，可以有效應用於組織工程之細胞分子療法。

關鍵字

口腔鱗狀細胞癌 ； 薑黃素 ； 微乳液 ； 超音波 ； 控釋

並列摘要

There is increasing interest in using natural products as anticancer agents, as many have antioxidative properties that may help to prevent cellular damage that can lead to cancer. In this study we determined the compositions, soybean lecithin, soybean oil and Tween 80, of curcumin-containing microemulsions which are stable after 40 days in a freezer. Moreover, the ingredients are all biocompatible, to reduce the side effects. The stable microemulsions were then characterized by their mean droplet size and zeta potential of 46 nm and -38 mV, respectively. Low frequency ultrasound was applied to curcumin microemulsions to maintain the effective concentration for the chemotherapy. It was found that the more aggressive oral squamous cell carcinoma cell lines have lower cellular viability. The ultrasound-enhanced delivery of curcumin as a cytotoxic agent is appealing, as the microemulsion could be ingested orally and the concentration adjusted so as to have little effect on healthy tissues in the absence of the ultrasound release trigger. Additionally, the in vitro controlled release of drugs may be useful in cellular molecular therapy in tissue engineering.

並列關鍵字

oral squamous cell carcinoma (OSCC) ； curcumin ； microemulsion ； ultrasound ； controlled release