Virulence of Native Isolates of Metarhizium anisopliae and Beauveria bassiana Against Helicoverpa armigera in Nepal

黑殭菌(Metarhizium anisopliae)與本土白殭菌(Beauveria bassiana)之本土分離株對蕃茄夜蛾(Helicoverpa armigera)之毒性於尼泊爾之研究

Jhalendra Prasad Rijal ； Yubak Dhoj GC ； Resham Bahadur Thapa ； Lekhnath Kafle

《台灣昆蟲》 28卷1期 (2008/03) Pp. 21-29

https://doi.org/10.6661/TESFE.2008003

摘要

在尼泊爾，於實驗室條件下，將蟲生真菌－黑殭菌(Metarhizium anisopliae)與本土白殭菌(Beauveria bassiana)的本土分離株對於蕃茄夜蛾(Helicoverpaarmigera)三齡幼蟲的致死率、感染率及LT50進行評估。加上4個黑殭菌分離株與2個本土白殭菌分離株，黑殭菌M1與本土白殭菌B3是所有分離株在濃度10^7 conidia/ml下毒性最強者。M1與B3分離株具有最高的致死率，且可用最短時間殺死蕃茄夜蛾幼蟲。於施用M1與B3分離株十天後，幼蟲致死率超過85%。在尼泊爾欲善加利用本土毒性分離株－黑殭菌M1與本土白殭菌B3，以達最佳功效，這些數據可作為未來研究的基礎。

關鍵字

本土白殭菌 ； 黑殭菌 ； LT50 ； 番茄夜蛾

並列摘要

The native isolates of entomopathogenic fungi, Metarhizium anisopliae and Beauveria bassiana, were evaluated against the third instar larvae of the chickpea pod borer, Helicoverpa armigera, for mortality, infection rate and LT50 under laboratory conditions in Nepal. Out of four isolates of M. anisopliae and two isolates of B. bassiana evaluated, M. anisopliae M1 and B. bassiana B3, were the most virulent ones of all isolates evaluated, with a concentration at 10^7 conidia/ml. The M1 and B3 isolates had the highest mortality rates and required the shortest time to kill the larvae of H. armigera. After treatment with M1 and B1 isolates for 10 days, the larval mortality exceeded 85%. These data lay the ground work for further studies in optimizing the effectiveness of indigenous virulent isolates, M. anisopliae M1 and B. bassiana B3 against H. armigera in Nepal.

並列關鍵字

Beauveria bassiana ； Metarhizium anisopliae ； LT50 ； Helicoverpa armigera