- 學位論文
茶菁經小綠葉蟬叮咬前後蛋白質體學及香氣成份變化之研究
Proteomic Research and Volatile Compounds Analysis of Tea Leaves with Feeding Damage by Jacobiasca formosana Paoli
摘要
椪風茶又名東方美人茶，為台灣特有生產之茶類，具有特殊之蜜香味及果香味，其原料來自於被小綠葉蟬—smaller green leafhopper Paoli ( Jacobiasca formosana Paoli ) 所叮咬過之茶菁。已知必須是經過小綠葉蟬叮咬之茶菁，才能製成風味良好之椪風茶。為了探究小綠葉蟬叮咬茶菁對茶樹造成之生理變化與椪風茶品質之可能影響，因而進行本研究。在蛋白質體學研究方面，以2D-PAGE電泳分析，我們發現受小綠葉蟬叮咬後的茶菁中，有四個蛋白質之表現量明顯增加，經蛋白質資料庫比對，它們分別為heat shock protein，superoxide dismutase，glyoxalase，以及thiosulfate sulfurtransferase (rhodanese)，而這些蛋白質或酵素，已知分別與植物之防禦或是逆境耐性之產生機制有關。經GC/MS分析茶菁的芳香物質，受小綠葉蟬叮咬後的茶菁含有明顯之 benzaldehyde, 3-carene, linalool, linalool oxides, linalool derivatives, benzyl nitrile, indole,alpha-farnesene, methyl salicylate, benzoic acid及cis-jasmone 等成分，推測這是椪風茶具有特殊香味之來源之一。綜合以上結果得知，小綠葉蟬叮咬茶樹並吸食芽葉韌皮部汁液，對茶樹造成逆境，因而引發茶菁之相關防禦機制，而這些揮發性物質則可能扮演訊息傳導、忌避害蟲，或是吸引害蟲天敵之角色。
並列摘要
Pong-Fong tea, also named Oriental Beauty tea, a unique tea only produced in Taiwan, is famous for the honey-like and ripen-fruit-like aroma. To use the tea leaves infested by a kind of small tea insect, smaller green leafhopper (Jacobiasca formosana Paoli), as the tea making material is the characteristic in production of Pong-Fong tea. Therefore, the feeding damage on the tea leaves is thought the key effect to the aroma formation of Pong-Fong tea. In the proteomic analysis, the elevated expression of four proteins was observed, including a heat shock protein, a superoxide dismutase, a glyoxalase, and a thiosulfate sulfurtransferase, which has been reported to be involved in plant stress or defense reactions. Through the volatile compounds analysis by GC/MS, we found that tea leaves with feeding damage by J. formosana Paoli contained more benzaldehyde, 3-carene, linalool, linalool oxides, linalool derivatives, benzyl nitrile, indole, alpha-farnesene, methyl salicylate, benzoic acid and cis-jasmone. It is concluded that the feeding damage in tea leaves by the phloem feeding insect, J. formosana Paoli, resulted to their stress and defense response, and the volatile compounds were emitted probably for signaling, herbivore deterrence and attraction for the predators or parasitoids of the herbivore.
參考文獻
延伸閱讀
- 黃志煜、吳志鴻、張振生、葉永廉、張上鎮（2003）。製程與採收季節對茶葉抽出物抗氧化活性之影響。國立臺灣大學生物資源暨農學院實驗林研究報告，17(4)，231-237。https://doi.org/10.6542/EFNTU.200312_17(4).0004
- 王志瑄、鄧資新（2018）。紅茶製程中茶菁採摘方法、發酵時間、溫度與烘焙溫度對茶湯活性物質含量與品質之影響。農林學報，66(3,4)，129-139。https://doi.org/10.30089/JAF.201812_66(3_4).0001
- 蔡志賢、王自存、張唯勤（2002）。包種茶萎凋與攪拌製程中茶菁之乙烯與二氣化碳生成及多酚氧化酶活性的變化。中國園藝，48(3)，227-238。https://doi.org/10.6964/JCSHS.200209.0227
- 謝中維（2015）。A Study of the Application of Spectrometer for the Detection of Pesticide Residues in Tea〔碩士論文，國立暨南國際大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0020-1008201519491400
- Chen, W. C., & Chen, C. P. (2020). Soil Properties and Soil Bacterial Diversity in Conventional and Converted Organic Tea Plantations. 國立臺灣大學生物資源暨農學院實驗林研究報告, 34(4), 349-364. https://doi.org/10.6542/EFNTU.202012_34(4).0005