Muscle Type from which Satellite Cells are Derived Plays a Role in their Damage Response

Chuang-Yu Lin ； Chun-Yin Hou ； Chung-Min Tsai ； Hsi Chang

《The Chinese Journal of Physiology》 63卷3期 (2020/06) Pp. 113-121

https://doi.org/10.4103/CJP.CJP_98_19

摘要

The aim of this study was to evaluate the response of satellite cells to muscular atrophies which possess different pathological characteristics and which were induced by distinct damages. Right lower limbs of rats were exposed to denervation or disuse and later its tibialis anterior (TA) or soleus (SOL) muscles were analyzed. After confirming their functional impairments indicated by common but distinct pathological and electrophysiological characteristics, the quantitative polymerase chain reaction analysis of Pax7 and Pax3 expressions and the number of Pax7^(+ve) and Pax3^(+ve) cells were analyzed sequentially at day 0, day 7, and day 14. TA muscles of both denervation-and disuse-induced atrophy models showed persisted low level of Pax7 expression from day 7 (0.91 ± 0.23 and 0.31 ± 0.07, P = 0.06, n = 6) through day 14 (1.09 ± 0.15 and 0.4 ± 0.09 [P ＜ 0.05]). On the other hand, significant elevations were observed in Pax3 expression in both atrophy models (2.73 ± 0.46 and 2.75 ± 0.26 [P ＜ 0.05]) at day 7. Similar to TA muscle, resembled pattern of Pax7 and Pax3 expression changes were observed between the SOL muscles of denervation-and disused-atrophy models. These trends were further confirmed by the changes in Pax7^(+ve) and Pax3^(+ve) cell numbers of TA and SOL muscles in both atrophy models. Despite the distinct pathological findings, similar patterns in the changes of Pax3 and Pax7 expressions and the changes of Pax7^(+ve) and Pax3^(+ve) cell numbers were observed between the denervation-and disuse-induced atrophy models and this commonality was admitted among the muscle type. Therefore, we claim that the muscle regeneration orchestrated by satellite cells was governed by the muscle type in which satellite cells reside.

關鍵字

Denervation-induced atrophy model ； disuse-induced atrophy model ； muscle regeneration ； muscle type ； Pax3 and Pax7

參考文獻

Mauro A. Satellite cell of skeletal muscle fibers. J Biophys Biochem Cytol 1961;9:493-5.

Weissman IL, Anderson DJ, Gage F. Stem and progenitor cells: Origins, phenotypes, lineage commitments, and transdifferentiations. Annu Rev Cell Dev Biol 2001;17:387-403.

Machida S, Booth FW. Regrowth of skeletal muscle atrophied from inactivity. Med Sci Sports Exerc 2004;36:52-9.

Degens H, Alway SE. Control of muscle size during disuse, disease, and aging. Int J Sports Med 2006;27:94-9.

Taniguchi Y, Sugenoya J, Nishimura N, Iwase S, Matsumoto T, Shimizu Y, et al. Contribution of central versus sweat gland mechanisms to the seasonal change of sweating function in young sedentary males and females. Int J Biometeorol 2011;55:203-12.