分子信籤感測非核酸分析物

A Non-Watson-Crick Base Pairing-based Molecular Beacon for the Sensing of Non-nucleic Acid Analytes of Interest

汪建興(Chien-Hsing Wang) ； 游俊國(Jyun-Guo You) ； 曾韋龍(Wei-Lung Tseng)

《化學》 75卷1期 (2017/03) Pp. 79-90

https://doi.org/10.6623/chem.2017007

摘要

分子信籤是一種短且具有單股、髮夾彎結構及修飾有螢光團及消光團的寡核苷酸。以Waston-Crick鹼基配對為基礎之分子信籤目前被廣泛應用於“Turn-on＂螢光偵測生物體液內目標DNA與即時聚合酶連鎖反應不同階段的產物。近年來，許多研究學者致力於開發以non-Watson-Crick鹼基配對的分子信籤，其能夠應用於高靈敏度及高選擇性偵測重金屬離子、硫醇分子、酵素-受質反應、硫酸多醣、與環二腺苷酸。本文詳細介紹及討論以non-Watson-Crick鹼基配對為基礎之分子信籤感測機制。

關鍵字

分子信籤 ； 硫酸多醣 ； 環二腺苷酸

並列摘要

Molecular beacon (MBs) are short, single-stranded, hairpin-shaped, and fluorophore-/ quencher-labeled oligonucleotide. They are intensively used for fluorescence turn on detection of target DNA in biological fluids and real-time polymerase chain reaction based on Watson-Crick base pairing. Recently, researchers have also devoted to developing non-Watson-Crick base pairing-based MBs for sensitive and selective detection of heavy metal ions, thiol molecules, enzyme-substrate reaction, sulfated polysaccharides, and cyclic diadenosine monophosphate. In this review article, non-Watson-Crick base pairing-based MBs are introduced and discussed their sensing mechanism in detail.

並列關鍵字

Molecular beacon ； Sulfated polysaccharides ； Cyclic diadenosine monophosphate