含銅氧化亞氮還原酶及其生化擬態化合物之歷史回顧與展望

Research Developments on the Copper Nitrous Oxide Reductase and Its Biomimetic Complexes

張育綸(Yu-Lun Chang) ； 許智能(Sodio C. N. Hsu)

《化學》 77卷4期 (2019/12) Pp. 277-288

https://doi.org/10.6623/chem.201912_77(4).005

摘要

氧化亞氮還原酶（nitrous oxide reductase; N_2OR）在厭氧呼吸過程的脫氮細菌中扮演相當重要的角色。氧化亞氮還原酶的晶體結構顯示出包含Cu_A與Cu_Z中心，Cu_A其負責電子傳遞，Cu_Z則是催化的活性中心：由四個銅與硫組成的團簇結構。酵素的銅硫團簇活性中心會催化氧化亞氮還原成氮氣，N_2O+2e^-+2H^+→N_2+H_2O。氧化亞氮還原酶催化的反應機制尚未非常清楚，透過仿生生物啟發的方法，可以讓化學家從模型化合物的結構及催化活性角度研究。這些方法有助於科學家們更加了解氧化亞氮還原酶的反應機制。

關鍵字

氧化氧氮還原酶 ； 銅硫團簇化合物 ； 仿生模型化合物

並列摘要

Nitrous oxide reductase (N_2OR) is a key enzyme in the anaerobic respiratory pathway of denitrifying bacteria. Based on the crystallographic study of N_2OR protein, the active center exhibits a Cu_A electron-transfer center and the Cu_Z catalytic center for the function of nitrous oxide reduction. The Cu_Z center is a copper-sulfur cluster contains four copper and one sulfur and could convert nitrous oxide to dinitrogen gas; N_2O+2e^-+2H^+→N_2+H_2O. The detail catalytic mechanism of N_2OR still is not fully understood yet. Biomimetic and bio-inspired model approaches could help chemists to evaluate and explore the structure and catalytic activity of modeling compounds of N_2OR. These approaches also help scientists to understand the detail reduction mechanism of nitrous oxide reductase.

並列關鍵字

Nitrous oxide reductase (N_2OR) ； Copper-sulfur cluster ； Bio-inspired model complexes