深層培養羊肚菌(Morchella esculenta)之最適化培養液組成

Optimization of the medium components for Morchella esculenta submerged culture

劉梅玉(Mei-Yu Liu)

指導教授 ： 李繡鈴

大同大學/工程學院/生物工程學系所/碩士(2005年)

摘要

本研究乃在探討液態培養M. esculenta BCRC 36348以獲得其多醣之最適培養液組成。當以glucose為碳源時，其菌絲體乾重、胞內多醣及總多醣產量最高，添加濃度為2 %時，可獲得最高之總多醣產量及產率。yeast extract為培養M. esculenta BCRC 36348 生產多醣之最適氮源，添加0.5 %yeast extract時，可獲得最高之總多醣產量及產率。培養液中添加植物油雖會抑制M. esculenta BCRC 36348之生長，但會顯著提高其總多醣產量、含量及產率，以添加1 % 玉米油之效果最佳。當以2 % glucose、0.5 % yeast extract、1 % corn oil、0.0068 % KH2PO4 及10 % mineral solution之培養液於25℃下進行M. esculenta BCRC 36348之液態培養6天後，可獲得最高總多醣之產量及含量，分別為2.18 g/L及335.64 mg/g DW。經由反應曲面試驗設計得知，當培養液組成為3.31 % glucose、0.39 % yeast extract、 1 % corn oil、0.0068 % KH2PO4 及10 % mineral solution時，培養M. esculenta BCRC 36348 6天後，可獲得最大之胞內多醣產量，約為1.97 g/L。若培養液組成為3.34 % glucose、0.41 % yeast extract、1 % corn oil、0.0068 % KH2PO4 及10 % mineral solution時，則可獲得最大之總多醣產量，約為2.22 g/L。

關鍵字

胞內多醣 ； 胞外多醣 ； 羊肚菌 ； 食藥用菇

並列摘要

In this study, the optimized medium composition for polysaccharide production in submerged cultures of M. esculenta BCRC 36348 were investigated. When using 2% glucose as carbon source, maximal mycelium dry weight, formation of intracellular polysaccharide and total polysaccharide were obtained. Yeast extract is the optimal nitrogen source. When 0.5% yeast extract were added, maximal production and yield of total polysaccharide were found. Although addition of plant oils inhibit the growth of M. esculenta BCRC 36348, production, content and yield of total polysaccharide significantly increased. Addition of 1% corn oil to the medium gave the best effect. After cultivation for 6 d, the maximum production and content of total polysaccharide, i.e. 2.18 g/L and 335.64 mg/g DW, respectively, by M. esculenta BCRC 36348 were found in the medium containing 2 % glucose, 0.5 % yeast extract, 1 % corn oil, 0.0068 % KH2PO4 and 10 % mineral solution.
With response surface methodology, maximal production of intracellular polysaccharide (1.97 g/L) was obtained by M. esculenta BCRC 36348 after 6 d of cultivation when the medium contained 3.31 % glucose, 0.39 % yeast extract, 1 % corn oil, 0.0068 % KH2PO4 and 10 % mineral solution. While maximal production of total polysaccharide (2.22 g/L) was found when medium contained 3.34 % glucose, 0.41 % yeast extract, 1 % corn oil, 0.0068 % KH2PO4 and 10 % mineral solution.

並列關鍵字

Morchella esculenta ； polysaccharide

參考文獻

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