Title

ANTIMICROBIAL, ANTIVIRAL AND CYTOTOXIC ACTIVITIES OF SELECTED MARINE ORGANISMS COLLECTED FROM THE COASTAL AREAS OF MALAYSIA

DOI

10.6119/JMST.2018.02_(1).0012

Authors

Yik Sin Chan;Cheong Wei Ong;Bee Ling Chuah;Kong Soo Khoo;Fook Yee Chye;Nam Weng Sit

Key Words

antibacterial ; antifungal ; seaweed ; sponge ; soft coral

PublicationName

Journal of Marine Science and Technology

Volume or Term/Year and Month of Publication

26卷1期(2018 / 02 / 01)

Page #

128 - 136

Content Language

英文

Chinese Abstract

Many marine organisms have developed the capability of producing unique metabolites and thus are highly likely to contain anti-infective agents. This study was conducted to investigate extracts of three seaweeds (Caulerpa racemosa, Caulerpa sertularioides, Kappaphycus alvarezii), two soft corals (Lobophytum microlobulatum, Sarcophyton auritum) and a marine sponge (Spheciospongia vagabunda) collected from Malaysian coast for antibacterial, antifungal, antiviral and cytotoxic activities. The samples were subjected to sequential solvent extraction in order to obtain hexane, chloroform, ethyl acetate, ethanol, methanol and water extracts. The antibacterial and antifungal activities were studied using a colorimetric broth microdilution method. The hexane extract of L. microlobulatum had the strongest antibacterial activity and exhibited the lowest minimum inhibitory concentration (0.04 mg/mL) and minimum bactericidal concentration (0.08 mg/mL) against Staphylococcus aureus and Bacillus cereus, respectively. For antifungal activity, the lowest MIC and minimum fungicidal concentration values were produced by the hexane extract of S. auritum against the dimorphic yeast Cryptococcus neoformans, both with 0.04 mg/mL. None of the extracts were active against the filamentous fungus Aspergillus fumigatus. Only the hexane and ethanol extracts of L. microlobulatum and the ethyl acetate extract of S. auritum exhibited strong inhibition on the cytopathic effect induced by the Chikungunya virus (a re-emerging mosquito-borne virus) with 50% effective concentrations of 14.3 ± 0.2, 124.3 ± 1.9 and 176.6 ± 9.7 μg/mL, respectively. Extracts from the two soft corals, L. microlobulatum and S. auritum possessed stronger antibacterial, antifungal and antiviral activities compared to the seaweeds and the sponge.

Topic Category 基礎與應用科學 > 海洋科學
工程學 > 市政與環境工程