由苦瓜硬化病果上所分離之矮南瓜嵌紋病毒鞘蛋白基因之分析

Sequence Analysis of the Coat Protein Gene of Zucchini Yellow Mosaic Virus Isolated from Diseased Woody Fruit of Bitter Gourd

黃金興( C.H Huang) ； 林詩舜( S. S Lin) ； 葉錫東( S. D Yeh)

《植物病理學會刊》 10卷1期 (2001/03) Pp. 11-18

https://doi.org/10.6649/PPB.200103_10(1).0002

摘要

Diseased fruits of bitter gourd (Momordica charantia L.) collected from southern Taiwan were found with symptoms of necrosis and woodiness. The presence of Papaya ringspot virus type W (PRSV W) alone, PRSV W combined with Zucchini yellow mosaic virus (ZYMV), or PRSV W combined with Cucumber mosaic virus (CMV) in the diseased fruits were detected by indirect ELISA. ZYMV TW-PT5 isolated from the fruit of bitter gourd originated from Pingtung was further characterized at the molecular level. The coat protein gene of TW-PT5 was amplified by RT-PCR with specific primers and the amplified cDNA fragments were cloned and sequenced. The CP gene of TW-PT5 was compared with those of 11 other ZYMV isolates originated from Taiwan and other areas of the world. The results revealed that the CP genes of the four Taiwan isolates share the highest nucleotide identities (＞97.7%) and amino acid identities (＞98.3%) with those of TW-PT5; therefore, TW-PT5 was classified with them in genotype I. Moreover, amino acid positions 73, 102,109, and 149 of the CP of TW-PT5, where lysine, serine, arginine, and aspartic acid reside, respectively, were found identical with those of the four Taiwan isolates of genotype 1. Phylogenetic analysis also indicated that TW-PTS is grouped in genotype I with the four Taiwan isolates, but distinct from a Taiwan isolate (TW-NTl) and four US isolates (genotype II), Reunion Island isolate (genotype III), and Singapore isolate (genotype IV).

關鍵字

苦瓜 ； 果實硬化 ； 矮南瓜黃化嵌紋病毒 ； 鞘蛋白 ； 序列分析

並列摘要

Diseased fruits of bitter gourd (Momordica charantia L.) collected from southern Taiwan were found with symptoms of necrosis and woodiness. The presence of Papaya ringspot virus type W (PRSV W) alone, PRSV W combined with Zucchini yellow mosaic virus (ZYMV), or PRSV W combined with Cucumber mosaic virus (CMV) in the diseased fruits were detected by indirect ELISA. ZYMV TW-PT5 isolated from the fruit of bitter gourd originated from Pingtung was further characterized at the molecular level. The coat protein gene of TW-PT5 was amplified by RT-PCR with specific primers and the amplified cDNA fragments were cloned and sequenced. The CP gene of TW-PT5 was compared with those of 11 other ZYMV isolates originated from Taiwan and other areas of the world. The results revealed that the CP genes of the four Taiwan isolates share the highest nucleotide identities (＞97.7%) and amino acid identities (＞98.3%) with those of TW-PT5; therefore, TW-PT5 was classified with them in genotype I. Moreover, amino acid positions 73, 102,109, and 149 of the CP of TW-PT5, where lysine, serine, arginine, and aspartic acid reside, respectively, were found identical with those of the four Taiwan isolates of genotype 1. Phylogenetic analysis also indicated that TW-PTS is grouped in genotype I with the four Taiwan isolates, but distinct from a Taiwan isolate (TW-NTl) and four US isolates (genotype II), Reunion Island isolate (genotype III), and Singapore isolate (genotype IV).

並列關鍵字

bitter gourd ； woodiness ； ZYMV ； coat protein ； sequence analysis