Concentration of n-3 PUFA in Saponified Menhaden Oil by fractional Solvent Crystallization

本研究以兩階段低溫溶劑結晶法，濃縮皂化鯡魚油所得自由態脂肪酸(FFA)中之n-3多元不飽和脂肪酸(PUFAs)。以氰化甲烷(acetonirtile)作為第一階段濃縮的溶劑。當溶劑/FFA=60mL/g，在-40℃下24小時後，於液相濃縮液中可將EPA、DHA及n-3 PUFA由原來的15.45%及32.63%分別提升至28.94%、18.36%及60.92%，n-3 PUFA的回收率為90.26%。在第二階段濃縮中，以30%氰化甲烷及70%丙酮之混合物為溶劑。當溶劑/FFA=60 mL/g時，於-80℃下24小時後，可將EPA、DHA及n-3 PUFA分別進一步提升至33.85%、21.35%及70.77%，n-3 PUFA回收率為89.03%(總回收率為80.36%)。

關鍵字

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並列摘要

A two –stage solvent crystallization process was developed for the enrichment of n-3polyunsturated fatty acids (PUFAs) in free fatty acids (FFAs) derived from saponified menhaden oil. Acetonitrile was used as the solvent in the first stage of the concentration process. At a ratio of acetonitrile to FFAs of 60mL/g, after 24 h at -40℃,the EPA, DHA and total n-3PuFA contents in the liquid phase could be raised from 15.45%, 9.67% and 32.63% to 28.94%,18.36% and 60.92%, respectively; with a total n-3 PUFA yield of 90.26% In the second stage, a mixture of 30% acetonitrile and 70% acetone was used as the solvent. At a solvent to FFAs ratio of 60mL/g, after 24 h at -80℃, the EPA, DHA and total n-3 PUFA contents in the liquid phase could be further raised to 33.85%, 21.35% and 70.77%, respectively, with a total n-3PUFA yield of 89.03T(overall yield 80.36%).

並列關鍵字

Fractional crystallization ； EPA ； DHA ； n-PUFA ； Menhaden oil

國際替代計量

Concentration of n-3 PUFA in Saponified Menhaden Oil by fractional Solvent Crystallization

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