Indole-3-butyric Acid Suppresses the Activity of Peroxidase While Inducing Adventitious Roots in Cinnamomum kanehirae

吲哚丁酸(IBA)有效地促進牛樟發根，在IBA處理的組織內過氧化酶(POX)的活性明顯比對照組小。IBA處理的組織內較低的木質素含量和下降的POX活性相關。我們認為在不定根的誘發過程，IBA抑制POX活性可能導致組織細胞逆分化，繼而產生新根。我們亦從牛樟幼根選殖並定序一新的POX cDNA，CKPX3，連帶它的調控區域。CKPX3調控區內包括生長素對應元素(auxin response element)、木質化元素、病源入侵及抗逆境元素等。我們的研究認為CKPX3 調控區內的生長素對應元素可能在不定根形成過程中因外加IBA的調節，並透過組織細胞內生長素反應因子(auxin response factors)抑制POX基因的表現。

關鍵字

不定根；牛樟；吲哚丁酸；木質化；過氧化酶

並列摘要

Indole-3-butyric acid (IBA) effectively promoted rooting in Cinnamomum kanehirae. The peroxidase (POX) activity significantly decreased in the IBA-treated tissues as compared with the controls. The lower amounts of lignin content in IBA-treated cuttings is correlated with the decline of POX activity. We suggest that the inhibition of POX activity may lead to the redifferentiation processes induced by IBA, which produces the new root primordia during the formation of adventitious roots. In this investigation, we also cloned POX cDNA from the young roots. A full-length cDNA of the POX gene designated was cloned by 5' and 3' RACE. According to the analysis of the promoter elements of CKPX3, we found some elements related to auxin response, lignification, pathogen invasion and stress response. Our results suggest that CKPX3 may have auxin responsive elements within its promoter which are suppressed by auxin response factors that are regulated by exogenously applied IBA during the adventitious rooting.

並列關鍵字

Adventitious root ； Cinnamomum kanehirae ； Indole-3-butyric acid ； Lignification ； Peroxidase

國際替代計量

Indole-3-butyric Acid Suppresses the Activity of Peroxidase While Inducing Adventitious Roots in Cinnamomum kanehirae

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