牛樟抽出物及精油對樟芝菌絲及子實體生長之影響

EFFECTS OF THE EXTRACTS AND ESSENTIAL OIL FROM CINNAMOMUM KANEHIRAE ON THE GROWTH OF MYCELIUM AND FRUITING BODY OF ANTRODIA CINNAMOMEA

高郁婷(Yu-Ting Kao)

指導教授 ： 張上鎮

國立臺灣大學/生物資源暨農學院/森林學研究所/碩士(2004年)

https://doi.org/10.6342/NTU.2004.01747

摘要

牛樟 (Cinnamomum kanehirae Hay.) 為台灣本土特有樹種。天然林牛樟樹幹大都中空腐朽，而造成牛樟腐朽之菌種實為一種褐腐菌，亦即是樟芝 (Antrodia cinnamomea)。樟芝無法於人工栽培之條件下出菇，故為了採取樟芝子實體出售便盜伐牛樟，此等濫伐造成牛樟瀕臨滅絕。牛樟抽出物已被證實含有促進樟芝菌絲生長的有效成分，因此本試驗續將牛樟抽出物中，具有促進樟芝生長活性的乙酸乙酯可溶部，利用薄層層析及管柱層析技術進行成分之初步分離，並評估各次分離部對樟芝生長的影響。此外，也由具有促進樟芝菌絲生長功效之次分離部中鑑定出trans-p-Menthane-1,4-diol、(CH3(CH2)nCH2OH, n=20, 22, 24)、

關鍵字

樟芝 ； 抽出成分 ； 牛樟 ； 精油

並列摘要

Cinnamomum kanehirae is an endemic broadleaf tree species in Taiwan. Heartwood of C. kanehirae in natural forest was decayed by one brown rot fungus, which is Chang-chih (Antrodia cinnamomea). Chang-chih is a precious fungus used for traditional medicinal purpose as an antidote and hepatic medicine. Unfortunately, for collection of it’s fruiting body, C. kanehirae trees were cut down unlawfully. To protect this unique endemic tree species, the objectives of this study were to investigate the characteristics of C. kanehirae wood decayed by A. cinnamomea and to evaluate the effects of the extracts of C. kanehirae on the mycelial growth of A. cinnamomea. Preliminary investigations have proved that the extracts of C. kanehirae promoted the mycelial growth of A. cinnamomea. Because of the excellent mycelial growth promoting ability of ethyl acetate soluble fractions, in this study, we separated it further into several subfractions and then evaluated the effects of each subfraction on the mycelial growth of A. cinnamomea. Results showed that E4 subfraction was the most active one of ethyl acetate soluble fraction. We also identified six compounds, that are trans-p-Menthane-1,4-diol, (CH3(CH2)nCH2OH, n=20, 22, 24),

並列關鍵字

Antrodia cinnamomea ； Cinnamomum kanehirae ； essential oils ； extracts

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