飲食添加山苦瓜對內毒素誘發敗血症小鼠發炎反應之影響

Effects of Wild Bitter Gourd Treatment on Endotoxin-induced Sepsis Inflammatory Responses

邱碩宇(SHUO-YU CIOU)

指導教授 ： 趙哲毅

亞洲大學/健康學院/保健營養生技學系碩士班/碩士(2009年)

摘要

文獻指出山苦瓜(Momordica charantia Linn. var. abbreviata Ser) 中具有活化PPARα及PPARγ的成分，具有調節血脂、血糖和免疫調節之保健功效，故本研究擬探討山苦瓜之飲食添加對於敗血症小鼠發炎反應之影響，以腹腔注射Lipopolysaccharide(LPS) 誘發敗血症之動物模式，以抗發炎藥Pyrrolidinedithiocarbamic acid (PDTC) 作為正對照組，評估飲食添加山苦瓜對於敗血症動物生化代謝及相關發炎指標之影響。採用六週齡Balb/c 雄性小鼠，分成六組分別為：○1 正常組(N) ○2 敗血症組(S) ○3 敗血症+ PDTC 組(P)○4 敗血症+1% 山苦瓜組(L) ○5 敗血症+2 % 山苦瓜組(M) ○6 敗血症+10 %山苦瓜組(H)。實驗組分別餵食含低、中、高不同比例(1%、2%、10%) 之山苦瓜凍乾粉末四週，犧牲前除了正常組，各組均施打LPS 產生敗血症，而正對照組則先注射PDTC 後再施打LPS。實驗結果顯示飲食添加之山苦瓜之組別其體重及副睪脂肪組織重量顯著較各組輕，而血脂質、肝脂質(TG、Cholesterol 及NEFA) 與膽紅素也較敗血症組低。飲食添加山苦瓜之組別其Acyl-CoA oxidase 蛋白質表現量增加，血糖濃度上升接近正常值，又以添加10% 山苦瓜組最為顯著。與發炎反應相關之血液生化數值顯示，添加山苦瓜之組別，其GOT、GPT、Creatinine、C-RP、NO2 之濃度皆低於敗血症組，血清及脾臟之IL-1、IL-6、TNF-分泌量皆顯著低於敗血症組，並提升了IL-10之分泌量。此外NF-B、iNOS、COX-2 蛋白質之表現量則顯著低於敗血症組。
綜合上述結果發現，飲食添加山苦瓜能促進脂質代謝，減少脂肪囤積，並使敗血症之低血糖現象獲得改善。飲食添加山苦瓜活化體內PPAR進而降低NF-B、iNOS、COX-2 等蛋白質之表現量，發炎相關指標及促發炎細胞激素下降，使肝、腎、脾等臟器損傷程度降低，進而使體內之代謝趨於正常。因此山苦瓜之添加有助於減緩並改善LPS 誘發敗血症所帶來的負面影響。

關鍵字

山苦瓜 ； 敗血症 ； 抗發炎效應 ； 過氧化體增殖劑活化受器

並列摘要

A wild species of Momordica charantia, wild bitter gourd (Momordica charantia Linn.var. abbreviata ser., or WBG), is native to tropical areas of Asia. A recent study showed that the ethyl acetate extract of WBG significantly activates PPARα and PPARγ in vitro. It was also shown to have a lipids-lowering and anti-diabetic effects in vitro. This study was designed to investigate the effects of WBG supplement on biochemical parameters and inflammatory-related mediators in septic mice. All Balb/c mice were classified into six groups as follows: normal group (N), sepsis group (S), positive control group (P), and WBG supplement sepsis groups (L, M, H). The results of the study demonstrated that WBG supplement sepsis groups its serum and liver lipid concentrations (triglyceride,cholesterol and non-esterified fatty acids) were significantly lower than sepsis group. As well as prevents liver fat accumulation and body weight gain associated with sepsis. Besides, the inflammatory-related mediators such as GOT, GPT,creatinine, C-RP, NO2 were also lower than sepsis group. Significantly inhibited the elevation of serum and spleen cytokines (IL-1, IL-6 and TNF-α) concentrations in WBG supplement sepsis groups especially in 10% (H) group. As a result of PPARγ activation lead to reducing the NF-B, iNOS and COX-2 protein expression. These results suggest that WBG supplement is beneficial for reducing LPS-induced sepsis resulted from its anti-inflammatory and lipids-lowering effects by modulating PPARα and PPARγactivation.

並列關鍵字

Wild bitter gourd ； Sepsis ； Anti-inflammatory effects ； AND ； A ； PPARγ

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