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Identification of a Chloramphenicol Resistance Plasmid from Lactobacillus Reuteri

源自洛德因乳酸桿菌之抗氯黴素質體的鑑定

Abstracts


洛德因乳酸桿茵G4株具有高達256μg/mL抗氯黴素之最低抑制濃度。經過一連串包括翦除、電孔、雜合、及轉載等分析實驗,當中的一個7.0kb質體(pTC82),終於成功的被鑑定為是帶有抗氯黴素因子的所在。本實驗在電孔實驗時所使用之先天不帶質體菌株L. reuteri DSM 20016,為第一次被用於此方面實驗以作為“同種接受宿主”者,相信在日後有關洛德因乳酸桿菌抗藥質體的鑑定上,必能扮演極出色的“同種接受宿主”角色。本實驗也成功的自pTC82質體轉載並表現了一段3.6 kb Cm^r FspI核酸片段至Escherichia coli 5 α;這是自L. reuteri的內質體成功的轉載並表現了一段抗氯黴素因子至Escherichia coli的第一次。

Parallel abstracts


Lactobacillus reuteri G4 was resistant to chloramphenicol (Cm) with a minimum inhibitory concentration (MIC) of 256 μg/mL. Through a series of analyses including curing, electroporatlon, hybridization, and cloning experiment, a 7.0 kb plasmid (pTC 82) was successfully identified as a Cm^r plasmid, The naturally plasmidless L. reuteri DSM 20016 was for the first time used as a homologous recipient in an eletroporation process for antibiotic resistant (R)-plasmid recognition and was found to be an excellent candidate for the future use in this regard. A 3.6 kb FspI DNA fragment which encodes chloramphenicol resistance determinant from pTC 82 was also successfully cloned into E. coli DH5 α. This is the first success of cloning and expressing a Cm-resistance determinant from an indeginous plasmid of L. reuteri into E. coli.

Parallel keywords

Lactobacillus reuteri Plasmid

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