氣喘病兒嗜中性白血球吞噬功能與血清組織胺之相關關係

Correlation of Neutrophil Phagocytosis to Serum Histamine in Children with Asthma

劉文章(Wen-Jang Liu) ； 江季勛(Chi-Hsiun Chiang) ； 黃德揚(Teh-Yang Huang)

《Acta Paediatrica Sinica》 27卷1期 (1986/02) Pp. 26-33

摘要

This study was conducted with thirty-six children during asthmatic attacks and with fifty-two normal children who acted as age matched controls. Consent of the children's parents was obtained prior to any experimental procedures. Tests were performed to detect serum histamine concentrations, neutrophil phagocytic function and the presence of serum inhibitor in bloods drawn from each child. The presence of a serum inhibitor was indicated by suppression of the phagocytosis of the donors' neutrophils.
The mean concentration of serum histamine in 27 asthmatics was 1.34±0.97 ng/ml, and was 0.40±0.38 ng/ml in 52 normal controls. The difference in concentration levels between these two groups was not biostatistically significant (t=1.45, P＞0.1). However, an extremely wide range of serum histamine concentrations were found in the asthmatic group.
Three types of neutrophil phagocytic function tests (N. B. T. test) were performed in 39normal controls and 26 asthmatics. The first type, in normal controls, with pool human serum and N.B.T. reagent had a mean optic density (O.D) variable of only 0.031±0.016. The stimulant was added in the second type of test, and the O.D. elevated to 0.061±0.023. However, in the third type of test, where the medium contained 1ng/ml, 10ng/ml or 100ng/ml of histamine, the O.D. slightly declined to 0.56±0.023, 0.053±0.027 respectively.
The O.D. for the asthmatics was 0.032±0.015 in the first type of test, 0.055±0.018 in the second, and 0.051±0.022, 0.47±0.022 and 0.044±0.017 under the 3 conditions of the third type of test.
Although the difference in each pair of values between both groups was not biostatistically significant, a lower level of O. D. were nevertheless detected in the asthmatic group. The asthmatic patients were more susceptible of the neutrophil phagocytasis to histamine concentration change than were the normal controls. Although, the rate of decrease of the neutrophil phagocytic function was enhanced by H1-antihistamine, the phagocytic function was increased by H2-antihistamine.
There was not a high correlation between O.D. of the neutrophil phagocytic function and serum histamine levels (r=0.346, P＞0.05). However, after the stimulant was added, a close correlation was found (r=0.410, P＜0.05). The serum from 36 asthmatics revealed a more significant suppression of neutrophil phagocytic function than did the 19 normal controls. These results indicate that the defects in the neutrophil phagocytic function in asthmatics occur both intracellularly and extracellularly. These defects may be either a result of frequent and sustained high serum histaminemia or from an unidentified inhibitor located in the serum.

並列摘要

This study was conducted with thirty-six children during asthmatic attacks and with fifty-two normal children who acted as age matched controls. Consent of the children's parents was obtained prior to any experimental procedures. Tests were performed to detect serum histamine concentrations, neutrophil phagocytic function and the presence of serum inhibitor in bloods drawn from each child. The presence of a serum inhibitor was indicated by suppression of the phagocytosis of the donors' neutrophils.
The mean concentration of serum histamine in 27 asthmatics was 1.34±0.97 ng/ml, and was 0.40±0.38 ng/ml in 52 normal controls. The difference in concentration levels between these two groups was not biostatistically significant (t=1.45, P＞0.1). However, an extremely wide range of serum histamine concentrations were found in the asthmatic group.
Three types of neutrophil phagocytic function tests (N. B. T. test) were performed in 39normal controls and 26 asthmatics. The first type, in normal controls, with pool human serum and N.B.T. reagent had a mean optic density (O.D) variable of only 0.031±0.016. The stimulant was added in the second type of test, and the O.D. elevated to 0.061±0.023. However, in the third type of test, where the medium contained 1ng/ml, 10ng/ml or 100ng/ml of histamine, the O.D. slightly declined to 0.56±0.023, 0.053±0.027 respectively.
The O.D. for the asthmatics was 0.032±0.015 in the first type of test, 0.055±0.018 in the second, and 0.051±0.022, 0.47±0.022 and 0.044±0.017 under the 3 conditions of the third type of test.
Although the difference in each pair of values between both groups was not biostatistically significant, a lower level of O. D. were nevertheless detected in the asthmatic group. The asthmatic patients were more susceptible of the neutrophil phagocytasis to histamine concentration change than were the normal controls. Although, the rate of decrease of the neutrophil phagocytic function was enhanced by H1-antihistamine, the phagocytic function was increased by H2-antihistamine.
There was not a high correlation between O.D. of the neutrophil phagocytic function and serum histamine levels (r=0.346, P＞0.05). However, after the stimulant was added, a close correlation was found (r=0.410, P＜0.05). The serum from 36 asthmatics revealed a more significant suppression of neutrophil phagocytic function than did the 19 normal controls. These results indicate that the defects in the neutrophil phagocytic function in asthmatics occur both intracellularly and extracellularly. These defects may be either a result of frequent and sustained high serum histaminemia or from an unidentified inhibitor located in the serum.

並列關鍵字

neutrophil phagocytosis ； N. B. T. ； serum inhibitor