An Assessment of DNA Polymorphisms and Genetic Relationships of Casuarina equisetifolia using RAPD Markers

Kuen-Yih Ho ； Chern-Hsiung Ou ； Jenq-Chuan Yang ； Ju-Jing Hsiao

《Botanical Bulletin of Academia Sinica》 43卷2期 (2002/04) Pp. 93-98

https://doi.org/10.7016/BBAS.200204.0093

並列摘要

One hundred forty-two individual samples belonging to 12 native accessions of Casuarina equisetifolia grown in an international Provenance trial garden in Taiwan were studied for RAPD variation. Twelve primers were used and 89 polymorphic bands were scored. An analysis of molecular variance (AMOVA) revealed that the percentages of variance attributable to variation among and within provenances were 39.28% and 60.72%, respectively. When accessions of north and south hemispheres were treated as two separate groups, the variance components between groups, among provenances within groups, and among individuals within provenances were 2.42%, 37.55%, and 60.03%, respectively. The difference between north and south latitude groups was not significant (p=0.1738). The average Nei's gene diversity for a location of origin was 0.1473. Cluster analysis and principal coordinates analysis revealed two major groupings. One group comprised solely the Cairns accession of Australia and identifies C. equisetifolia var. incana while the other group comprised the remaining accessions of C. equisetfolia var. equisetfolia. The RAPD data support the taxonomic treatment of two varieties in this species. Some regional relationships were observed within C. equisetfolia var. equisetifolia. Study results indicated a large genetic variation among the native accessions of Casuarina equisetifolia. Therefore, the results of provenance trials are expected to be useful in the selection of a suitable provenance for a particular coastal environment.

並列關鍵字

Casuarina equisetfolia ； Genetic variation ； RAPD ； Provenance trial