Effects of Cryptococcus Laurentii (Kufferath) Skinner in Combination with Sodium Bicarbonate on Biocontrol of Postharvest Green Mold Decay of Citrus Fruit

羅倫隱球酵母與碳酸氫鈉結合使用對柑桔採後綠黴病的生物防治效果

張紅印(Hong-Yin Zhang) ； 傅承新(Cheng-Xin Fu) ； 鄭曉冬(Xiao-Dong Zheng) ； 何丹(Dan He) ； 單麗君(Li-Jun Shan) ； 詹喜(Xi Zhan)

《Botanical Bulletin of Academia Sinica》 45卷2期 (2004/04) Pp. 159-164

https://doi.org/10.7016/BBAS.200404.0159

摘要

本文測定了羅倫隱球酵母單獨使用或與碳酸氫鈉結合使用對柑桔綠黴病的防治效果。在馬鈴薯葡萄糖瓊脂固體培養基（PDA）體外抑菌實驗中，把生長有羅倫隱球酵母的營養酵母葡萄糖固體培養基（NYDA）切塊放置在塗布有指狀青黴孢子的馬鈴薯葡萄糖瓊指脂固體培養基上一起培養，羅倫隱球酵母不能抑制黴菌的生長；而在馬鈴薯葡萄糖液體培養基（PDB）體外抑菌實驗中，羅倫隱球酵母活細胞有效的抑制了黴菌孢子的萌發。在接種5 x 104個／毫升的指狀青黴孢子後，羅倫隱球酵母顯著地抑制了綠黴病的發展。無論柑桔在20°C下貯存5天或在2°C下貯存50天，拮抗酵母的濃度越高，綠黴病的發病率越低。碳酸氫鈉可以加強羅倫隱球酵母的抑菌效果。將碳酸氫鈉和羅倫隱球酵母結合使用可以取代殺菌劑用於柑桔採後綠黴病的防治。

關鍵字

羅倫隱球酵母 ； 碳酸氫鈉 ； 綠黴病 ； 柑桔 ； 生物防治。

並列摘要

The potential of using Cryptococcus laurentii alone or in combination with sodium bicarbonate solution for control of Penicillium digitatum (green mold) on oranges was investigated. Agar disks of C. laurentii NYDA cultures placed on PDA plates seeded with pathogen did not inhibit the growth of P. digitatum. Spore germination of P. digitatum in PDB was significantly controlled in the presence of living C. laurentii cell suspensions. Cryptococcus laurentii significantly controlled green mold on oranges after challenge with 5 × 104 spores/ml of P. digitatum. The higher the concentrations of the antagonist, the lower the disease incidence regardless of whether the fruit was stored at 20°C for 5 days or 2°C for 50 days. The efficacy of C. laurentii for control of green mold was improved when combined with sodium bicarbonate. The combination of sodium bicarbonate and C. laurentii could be an alternative to fungicides for control of postharvest green mold disease on citrus fruits.

並列關鍵字

Biocontrol ； Citrus fruits ； Cryptococcus laurentii ； Green mold ； Sodium bicarbonate