- 期刊
由嗜菊短頭脊沫蟬泡沫液中分離之拮抗細菌對植物病原真菌之抑菌效用
Antifungal Effect of Antagonistic Bacteria Isolated from the Froth of Spittlebug Poophilus costalis Against Phytopathogenic Fungi
摘要
沫蟬若蟲的泡沫液在田間未見有真菌發霉現象，據此觀察，由台灣的嗜菊短頭脊沫蟬［Poophilus costalis（Walker, 1851）］之泡沫液分離拮抗細菌，並針對此類細菌進行對植物病原真菌生長之抑制效果及幾丁質酶活性分析。本研究由沫蟬泡沫液中分離出2種細菌對豌豆鐮孢菌（Fusarium oxysporum f. sp. pisi）與芒果炭疽病菌（Colletotrichum gloeosporioides Penz）之生長有明顯抑制作用，並經由16S rDNA及幾丁質酶基因序列鑑定AY與DT分別為Stenotrophomonas maltophilia與Delftia tsuruhatensis。其中已確認S. maltophilia AY可產生外幾丁質酶：N-乙醯葡萄糖胺酶與幾丁二醣酶，以及內幾丁質酶，其比活性各為1.1、19.9及19.1 nmol p-nitrophenol min^(-1) mg^(-1) protein。這2種拮抗細菌未來在植物病害防治上具有發展潛力。
並列摘要
For no fungi growing on the froth of spittlebug in field, the antifungal effect and chitinase activity of the bacteria isolated from the froth of Poophilus costalis (Walker, 1851) were analyzed in this study. Two isolates of bacteria had high antagonistic activities against Fusarium oxysporum f. sp. pisi and Colletotrichum gloeosporioides Penz were identified as Stenotrophomonas maltophilia AY and Delftia tsuruhatensis DT by 16S rDNA and chitinase gene sequences. S. maltophilia AY produced 1.1, 19.9, and 19.1 nmol p-nitrophenol min^(-1) mg(-1) protein of exochitinase β-N-actylglucosaminidase, chitobiosidase and endochitinase, respectively. These two antagonistic bacteria may have the application potential on pathogenic control in the future.
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