台灣地區養殖魚類抗生類殘留之調查

An Investigation of Antibiotic Residues of Cultured Fishes in Taiwan

張翠瑛(Tsui-Ying Chang) ； 黃琬惟(Wian-Wei Huang) ； 朱淑儀(Shu-Yi Chu) ； 郭淑寬(Shu-Kuan Kuo) ； 洪其璧(Chi-Byi Horng)

《藥物食品檢驗局調查研究年報》 7號 (1989/09) Pp. 122-130

https://doi.org/10.6946/ASRNL.198909.0122

摘要

民國75年11月至76年5月自台北市、高雄市及台灣省北、中、南、東等四區之傳統市場、超級市場抽購養殖魚類270件（包括鰻魚98件、鯉魚科43件、鱸鱒魚42件、虱目魚45件、吳郭魚42件）檢驗氯黴素、羥四環素、氯四環素等三種抗生素，結果氯黴素、羥四環素均未檢出，氯四環素檢出4件（鰻魚2件、鱸魚1件、虱目魚1代），檢出率1.5%。
四環素類之試驗微生物為Bacillus cereus var. mycoades ATCC 11778以圓筒平板法及生物自析法定性定量，檢出限量羥四環素為0.05ppm，氯四環素為0.01ppm，平均回收率分別為90.2%及84.5%；氯黴素之試驗微生物為Escherichia coli NIHJ，以圓筒平板法及液相層析法定性定量，檢出限量1.25ppm，液相層析最低感度可達1ng，平均回收率89.3%。

關鍵字

台灣地區 ； 養殖魚類 ； 氯黴素 ； 羥四環素 ； 氯四環素 ； 高效液相層析儀 ； 圓筒平板法 ； TLC-生物自析法

並列摘要

From Nov. 1986 to May 1987. 270 cultured fishes (including 98 eels, 43 cyprinids, 42 perciformes and Rainbow Trout, 45 milk fishes, 42 sarotherdons) were purchased from the fresh markets and supermarkets in Taiwan area and analyzed for oxytetracycline (OTC), chlorotetracydine (CTC), chloramphenicol (CP). The results showed that OTC and CP were undetected in all samples and CTC was detected in 4 sample which included: 2 eels, 1 perciform, 1 milk fish.
The cup-and-plate method and TLC-bioautography with Bacillus subtilis varmycoides ATCC 11778 as the test organism were employed for the TCs assay and the average recoveries were 90.2% (OTC) and 84.5% (CTC). As little as 0.05 pm (OTC) or 0.01 ppm (CTC) was detectable.
The cup-and-plate method with E. coli NIHJ as the test organism and HPLC were employed for the CP assay and the average recovery was 89.3%. As little as 1.25 ppm was detectable by bioassay. The detecting limit by HPLC was 1 ng.

並列關鍵字

Taiwan ； Cultured fishes ； Chloramphenicol ； Oxytetracycline ； Chlorotetracycline ； HPLC ； Cylinder-plate method ； TLC-bioautography