The Role of Serum-Supplements in Organ Culture for Pre-Damaged Corneal Endothelium

血清添加物對於內皮細胞預受破壞之角膜於器官培養中之影響

林昌平(Chang-Ping Lin) ； Matthias Bohnke ； Jorg Draeger

《The Kaohsiung Journal of Medical Sciences》 6卷8期 (1990/08) Pp. 436-439

https://doi.org/10.6452/KJMS.199008.0436

摘要

將36個豬角膜置於0.18％食塭水中震盪，散佈性的破壞角膜內皮細胞。然後將 10個角膜置於基本培養液（MEM）添加10％胎牛血清( FCS ; FCS組）；另10個角膜置於MEM添加10 % Nu-serumtm( Nu組）；另16個角膜只供應 MEM 為對照組。角膜於32°C，5 % CO2，95％相對溼度之培養箱中，以器官培養法培養一週。比較兩組血清添加組之細胞密度及形態積分均無差異，為防止血清引發過敏反應之可能性及考慮血清添加物之穩定性，可考慮以Nu-serum 取代胎牛血清為角膜器官培養液之添加物。經染色後，三組之大部份角膜均保持了相當完整之內皮層，顯示內皮層雖然培養前預受破壞，而於器官培養當中，不論培養液有無血清添加物，其內皮細胞均可再生修護。而比較有無血清添加物組之細胞密度，分別為 2454±450，2776±476和2627±558 cells/mm2，統計學上其差異無慧義，而形態積分也無明顯差異，因此血清添加物於中短期器官培養保存法之效益相當有限，其添加之必要性尚待更進一步之研究。

並列摘要

Thirty-six pig corneas were shaken in 0.18% NaCl to induce disseminated corneal endothelial damage. After treatment, the corneas were divided and cultured in 3 different combinations of media to study the effects of serum- supplements in corneal endothelial regeneration. There were MEM+1 0% FCS (FCS-group; n=10); MEM+10% Nu-Serum (Nu-group; n=10) and MEM only (control group; n=16). After one week of culturing, most of the corneas in all 3 groups, despite the pre-damage, kept intact endothelia. This means that the endothelia were repaired whether the sera were supplemented or not. The cell densities of each were 2,454± 450 cells/mm2, 2,776± 476 cells/mm2 and 2,627±558 cells/mm2 respectively. The differences between each group were statistically insignificant. The morphological findings were also without much difference. When considering the risk of serum induced hypersensitivity, FCS can be replaced by NuSerumTM in the organ culture medium for corneal preservation. Since the advantage of serum-supplements in medium-term organ cultures seems limited, the necessity of serum supplements should be further reevaluated.

並列關鍵字

fetal calf serum ； corneal endothelium ； regeneration.