氯烯類污染場址地下水中關鍵微生物與基因濃度之分佈

Distribution of Key Microbes and Functional Genes in the Chlorinated Ethene-Contaminated Groundwater

游漢威(Henry Yew) ； 莊蕙萍(Hui-Ping Chuang) ； 黃良銘(Liang-Ming Whang) ； 林財富(Tsair-Fuh Lin)

《農業工程學報》 63卷1期 (2017/03) Pp. 13-21

https://doi.org/10.29974/JTAE.201703_63(1).0003

摘要

含氯烯類化合物為地下水中最常見污染類別之一，近期所推崇具有競爭力的現地生物復育法，其關鍵因子為污染場址是否存在或適合培養出具有降解能力的微生物菌群。本研究針對台灣南部兩個受氯烯類污染場址，在37 口監測井進行採樣、並分析目標化學物質和微生物。微生物分析結果顯示，以SybrGreen-qPCR 法來定量樣本中總細菌和Dehalococcoides 屬與氯乙烯還原性脫鹵基因(vcrA gene)的豐富度，地下水中具還原脫氯能力的Dehalococcoides 屬約佔總細菌群的0.0001~14.58%，其遞增或遞減趨勢以總氯烯類濃度0.8 mg/L 為分界。而在厭氧還原脫鹵的過程中，負責中後段降解的vcrA 基因數量與Dehalococcoides 屬的豐富度呈正相關(R^2 = 0.72)，並與氯乙烯濃度呈現相關性。因此，本研究所評估的兩個污染場址為具有生物復育的潛力場址。

關鍵字

氯乙烯 ； Dehalococcoides 屬 ； 氯乙烯還原性脫鹵基因

並列摘要

Chlorinated ethenes are a group of chemicals commonly found in contaminated groundwater. In-situ bio-remediation is a popular and competitive technology for contaminated sites, and the one of the factors to influence the effectiveness of remediation is presence of degrading microbes in the sites. This study is aimed to detect the presence of targeted chemicals and microbes in 37 wells in two sites contaminated with chlorinated ethenes. Results of microbial analyses with SybrGreen-qPCR showed that genus Dehalococcoides, which had the ability to degrade chlorinated ethene, was 0.0001~14.58% of total bacteria, and total chlorinated ethenes of larger than 0.8 mg/L may inhibit the growth of genus Dehalococcoides. Abundance of vcrA gene, capable of degradation of chlorinated ethenes to ethene, is positive correlated with the abundance of genus Dehalococcides (R^2 = 0.72) and the concentration of chlorinated ethenes. The results of study suggested that the two contaminated sites studied have the potential for bio-remediation.

並列關鍵字

Chlorinated ethane ； Genus Dehalococcoides ； Reductive dehalogenase