Optimized Physical Parameters for High Cell Density Cultivation of the Indigenous Thiobacillus thiooxidans by Response Surface Methodology

以反應曲面法決定高密度培養本土性嗜酸性硫氧化硫化桿菌的最佳物理參數

藍彥文(Yann-Wen Lan) ； 劉宣良(Hsuan-Liang Liu) ； 鄭陽助(Yang-Chu Cheng)

《Journal of the Chinese Institute of Chemical Engineers》 36卷3期 (2005/05) Pp. 243-251

https://doi.org/10.6967/JCICE.200505.0243

摘要

本研究以反應曲面法決定高密度培養本土性嗜酸性硫氧化硫化桿菌之最佳物理參數。我們利用一組2^4中心混成法來決定培養時最佳的四個物理參數，包含溫度、搖瓶轉速、起始pH值、及接種濃度。實驗結果證實，在四個參數中，搖瓶轉速對微生物的產率有最大的影響。當這些參數以二階方程式迴歸所得到的值用於為生物的生長條件，可得到最佳的生長情況。微生物最佳的生長條件決定如下：溫度為31℃、搖瓶轉速為114 rpm、起始pH值為3.98、及接種濃度為9.6%。以這些條件所預測的最大乾菌重及硫酸產量分別為0.773 g/L及65,296 ppm。

並列摘要

In this study, response surface methodology (RSM) was employed to determine the optimal physical parameters for high cell density cultivation of the indigenous Thiobacillus thiooxidans. Optimization of four parameters (temperature, shaking rate, initial pH, and inoculum size) was attempted by using a 2^4 full-factorial central composite design (CCD) with a statistical method for experimental design and analysis. The experimental results shoed that the shaking rate had a more significant effect than the other three variables on the yield of the microorganism, and that an optimal growth level was achieved when these physical parameters were optimized by fitting a second-order polynomial model. The optimized values of the tested parameters for the maximal production of sulfuric acid were as follows: temperature=31℃, shaking rate=114 rpm, pH=3.98, and inoculum size=9.6%. The maximal predicted yields was achieved when the cell concentration and sulfuric acid production were 0.773 g/L and 65,296 ppm, respectively.

並列關鍵字

Response surface methodology (RSM) ； High cell density ； Thiobacillus thiooxidans ； Central composite design (CCD) ； Sulfuric acid