Disruption Kinetics of Bacterial Cells during Purification of Poly-β-Hydroxyalkanoate Using Ultrasonication

於純化聚羥基烷酯時使用超音波破碎細菌細胞之動力學

黃國楨(Kuo-Jen Hwang) ； 游少甫(Shao-Fu You) ； 董崇民(Trong-Ming Don)

《Journal of the Chinese Institute of Chemical Engineers》 37卷3期 (2006/05) Pp. 209-216

https://doi.org/10.6967/JCICE.200605.0209

摘要

聚羥基烷酯(PHA)是嗜塩菌之細胞內產物。為了獲得PHA，可以將嗜塩菌之細胞以超音波破碎，再以離心分離純化PHA。純化的結果以SEM進行觀察，發現多數PHA微粒的尺寸介於0.2至0.8微米之間。SEM的影像亦証明了超音波可以將嗜塩菌細胞有效地破碎。Kick的減積理論被用來描述在超音波破碎的過程中樣品平均尺寸的變化，所需的能量與減積比率成正比。本研究並基於所提出的減積機制來探討嗜塩菌細胞之破碎動力學，並且推導一理論模式來關聯細胞殘存分率、音波功率、粒徑分布指標與操作時間之間的關係。這個模式在沒有調整參數的情況下，可以提供一理論方式來預測各操作條件下之細胞破碎分率。結果顯示：提高音波功率或操作時間皆會降低細胞的殘存分率。使用這個理論模式，吾人可以選擇最適的音波功率與操作時間來達到特定的細胞破碎分率。

並列摘要

Poly-β-hydroxyalkanoate (PHA) is the intracellular product of Haloferax mediterranei In order to gain PHA. H. mediterranei cells are disrupted by an ultrasonication, and PHA is then purified by a centrifugal separation The results of purification are observed by SEM Most sizes of PHA granules range from 0.2 to 0.8 μm The SEM images demonstrate that the ultrasonication is an efficient mode for the cell disruption of H. mediterranei The Kick's size reduction la is used to describe the mean size variation during an ultrasonication operation The required energy is in proportional to the size reduction ratio Based on this size reduction mechanism, the disruption kinetics of H. mediterranei cells is studied and a theoretical model is derived to relate the cell survival fraction, acoustic power, size distribution index, and operation time This model provides a theoretical method to predict the cell disruption fraction by ultrasonication under various operating conditions with no parameter adjustment The results show that an increase in acoustic power or operation time leads to lower survival fraction of cells Use of this theoretical model, one can choose the optimum acoustic power and operation time to attain a specific cell disruption fraction

並列關鍵字

Cell disruption ； Ultrasonication ； Disruption kinetics ； PHA ； Bio-separation