Detection of Vibrio anguillarum and Vibrio alginolyticus by Randomly Cloned DNA Fragments

應用隨機基因探針偵測Vibrio anguillarum和Vibrio alginolyticus

謝玉玲(Yu-Ling Hsieh) ； 蔡珊珊(San-San Tsay)

《TAIWANIA》 44卷3期 (1999/09) Pp. 404-412

https://doi.org/10.6165/tai.1999.44(3).404

摘要

以Vibrio anguillarum和Vibrio alginolyticus作為測試菌株。利用限制酶HindIII和EcoRI將細菌的基因體DNA切割後，選出介於0.5-2.0 kb之片段選殖到質體pUC19上。利用非放射性探針標定篩選的片段，由102選殖的片段中找到一段只能與V. alginolyticus雜交；由94選殖的片段中找到三段只能與V. anguillarum雜交的片段，並找到一段只能與V. anguillarum血清型C作用的片段。

關鍵字

Vibrio anguillarum ； Vibrio alginolyticus ； 菌落雜交 ； 隨機選殖DNA片段 ； 血清型

並列摘要

Vibrio anguillarum and Vibrio alginolyticus were used as tested organisms. Restriction endonuclease HindIII and EcoRI digested DNA fragments from the above two bacteria were randomly selected and inserted into vector pUC19. Among 0.5-2.0 kb DNA fragments were recovered from agarose gel to prepare non-radioactive DIG-labeled probes. One out of 102 cloned fragments could hybridize only to V. alginolyticus. Three out of 94 cloned fragments could hybridize only to V. anguillarum and one fragment is specific for serotype C.

並列關鍵字

Vibrio anguillarum ； Vibrio alginolyticus ； Colony hybridization ； Randomly cloned fragments of DNA ； Serotype