- 期刊
Development of an efficient in vitro propagation protocol for Satureja punctata - A rare aromatic and medicinal plant
並列摘要
Satureja punctata is an important aromatic and medicinal plant in Lamiaceae family. This plant is threatened mainly due to overgrazing, deforestation, over harvesting of the whole plant for medicinal purpose. This study was aimed to develop an efficient in vitro propagation protocol for S. punctata using shoot tips. Shoots excised from in vitro germinated seedlings were used as mother plant for culture induction on MS medium supplemented with different concentrations of 6-benzyl amino purine (BAP) and Kinetin (KIN). Highest shoot induction frequency (100%) with highest mean number (8.25±1.64) of shoot per explant was obtained at 0.5 mg/L BAP. In multiplication media, among different concentrations of BAP, KIN, BAP×KIN and KIN×NAA (α-Naphthalene acetic acid), BAP × NAA found to be an optimum concentration with (26.20±2.71) mean number of shoots. The best shoot height (2.48 ± 0.13 cm) was achieved on growth regulators free medium, which was used as control. Half strength MS medium containing different concentrations of Indole-3- butyric acid (IBA), Indole-3-acetic acid (IAA) and NAA were used for root induction. The highest rooting percentage (100%), root number (5.90±0.48) and root length (1.55±0.11 cm) were attained in the presence of 1.0 mg/L IBA. Up on acclimatization, 97.5% survived plants were recorded. The results demonstrated that, this study was very important for mass propagation and ultimate conservation of this valuable plant.
參考文獻
延伸閱讀
- 沈榮壽、沈再木（1997）。In vitro Propagation of Furcraea foetida。中國園藝，43(1)，41-49。https://doi.org/10.6964/JCSHS.199703.0041
- 張淑華、蔡錦瑩、許原瑞、何政坤（2005）。Micropropagation and Evaluation of the Camptothecin Content of Camptotheca acuminata。臺灣林業科學，20(4)，331-340。https://doi.org/10.7075/TJFS.200512.0331
- 倪蕙芳、賴素玉、許淑麗、張淑芬、楊宏仁（2010）。Evaluation of Reproduction of Pratylenchus coffeae on Yam (Dioscorea spp.) in vitro Culture。台灣農業研究，59(1)，42-48。https://doi.org/10.6156/JTAR/2010.05901.04
- 吳倩芳、徐善德、沈再木（2003）。Studies on Somatic Embryogenesis and Regeneration of Polianthes tuberosa L.。中國園藝，49(2)，183-197。https://doi.org/10.6964/JCSHS.200306.0183
- Thiombiano, A. (2008). Life History and Host Specificity of Pareuchaetes pseudoinsulata on Chromolaena odorata in Quarantine Laboratory [master's thesis, National Pingtung University of Science and Technology]. Airiti Library. https://doi.org/10.6346/NPUST.2008.00260