Mycorrhizal fungi associated with Taiwanese Pyrola morrisonensis (Ericaceae) in a naturally regenerated forest

Yosuke MATSUDA ； Takashi UESUGI ； Tsai-Wen HSU ； Chien-Fan CHEN

《TAIWANIA》 62卷4期 (2017/12) Pp. 399-406

https://doi.org/10.6165/tai.2017.62.399

摘要

Pyrola morrisonensis, an evergreen herb in the family Ericaceae, is endemic to Taiwan. We examined mycorrhizal development and the associated fungi in this species. Nine plants were collected in a naturally regenerated forest in central Taiwan. The plants were genetically identical in their internal transcribed spacer (ITS) region, and their sequences matched the known sequence for P. morrisonensis. Fine roots of each plant were colonized by mycorrhizal fungi that formed mycorrhizas either with or without fungal mantles. DNA sequences of the ITS region of these fungi suggested that they belonged to mycorrhizal taxa that are common tree symbionts. Among them, members of Thelephoraceae were the dominant taxon in the host plants. These results indicate that P. morrisonensis is intimately associated with mycorrhizal fungi that might also connect with neighboring trees.

關鍵字

ITS ； Molecular identification ； Operational taxonomic unit ； Pyrola morrisonensis, root morphology ； Thelephoraceae

參考文獻

Chou, W.-N.and Y.-Z. Wang. 2005. Nine species of Russula (Basidiomycotina) new to Taiwan. Taiwania50(2): 93–100.http://doi.org/10.6165/tai.2005.50(2).93

Tschen, J. S. M., I. C. Ho, H. S. Hsu and E. F. C. Tschen. 2004. Distribution of macrofungi in the Quantaushi forest, a long-term ecological research site in Taiwan. Fung.Sci19: 1–19.

Altschul, S.F., T.L. Madden, A.A. Schäffer, J. Zhang, Z. Zhang, W. Miller and D.J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic acids Res. 25(17): 3389–3402.http://doi.org/10.1093/nar/25.17.3389

Edgar, R.C. 2010. Search and clustering orders of magnitude faster than BLAST, Bioinformatics 26(19): 2460-2461.http://doi.org/10.1093/bioinformatics/btq461

Freudenstein, J.V. 1999. Relationships and character transformation in Pyroloideae (Ericaceae) based on ITS sequences, morphology, and development. Systematic Bot. 24(3): 398–408.http://doi.org/10.2307/2419697