蘆筍花葯培養單倍植物體形成之研究

Studies on Anther Culture and Haploid Plant Regeneration of Asparagus

蔡新聲(Hsin-Sheng Tsay) ； 賴本智(Pen-Chih Lai) ； 紀迺淳(Nai-Chwen Chi)

《中華農業研究》 29卷4期 (1980/12) Pp. 309-319

https://doi.org/10.29951/JARC.198012.0009

摘要

含有2mg/1 NAA(Naphthalene acetic acid)，1mg/1 BA(N^6-Benzyladenine)，6% sucrose及將無機鹽類減半（Na-Fe-EDTA除外）之Murashige and Skoog (1962)培養基，可誘導蘆筍單核期的花葯產生極高百分率的花葯callus。不同品種間誘導callus形成的百分率差異極大。UC 309第一次花粉有絲分裂期所培養的花葯，黑暗處理者優於照光處理老。植株間花葯具有不同的callus形成力，這種株間巨大的差異可能是幾次試驗無法獲得一致結果的主要原因。
蘆筍花葯培養callus的形成，與花葯的褐化有明顯的相關（相關係數r=0.8046(上標 **)），大部分產生callus的花葯，都在培養後3週褐化；變成白色的花葯，形成callus機會極少。將誘得之花葯callus繼代培養於含有1mg/l NAA, 0.5mg/l BA之Murashige et al.(1972)培養基可誘導植物體產生。不同品種間的callus具有不同的分化能力。

並列摘要

Asparagus (Asparagus officinalis L.) anthers containing microspores in early-uninucleate to early-binuclate stages were successfully cultured in 1/2 strength Murashige and Skoog (1962) medium (except Na-Fe-EDTA) supplemented 2 mg/l NAA (Naphthalene acetic acid), 1 mg/l BA (Benzyladenine) and 6% sucrose for callus formation. Highest frequencies of callus induction were obtained from anthers with uninucleate microspores. Callus formation ability differed among varieties also among individual plants. Callus could be induced under either light or dark condition although better results were obtained when anthers at mitosis stage were cultured in darkness. Differences among individual plants of the same variety caused erratic results in this experiment. This was probably due to the genetic diversity of hybrid F, population.
In asparagus anther culture, there was a positive correlation between anther browning and callus formation (r=0.8046(superscript **)). The color of anthers turned to brown or white after 3 weeks in culture. Callus was hardly induced from anthers with a white-color appearance.
The organogenesis was induced when callus was subcultured to Murashige et al. (1972) medium with 1 mg/l NAA and 0.5 mg/l BA. The differentiation ability were different among the six varieties tested.