玉米幼苗乾物累積重與銨同化酵素活性間之相關

Relationships between Ammonium Assimilation Enzyme Activities and Dry Weight Accumulation of Maize Seedling

謝光照(Guang-Jauh Shieh) ； 曹文隆(Wen-Long Tsaur) ； 吳慧茹(Huey-Ru Wu) ； 盧煌勝(Hung-Shung Lu)

《中華農業研究》 52卷3期 (2003/09) Pp. 238-246

https://doi.org/10.29951/JARC.200309.0008

摘要

本試驗以玉米自交系和單雜交種各四個為材料，於溫室環境下，利用細砂為栽培介質，並於玉米不同株齡期進行50mM NH4(上标 +)溶液連續澆灌18天後，調查幼苗乾物累積重與其銨同化酵素活性之表現。結果顯示GS(glutamine synthetase)活性、地上部乾物累積重、根部乾物累積重、整株乾物累積重和地上部／根部比值呈現隨株齡之增大而增加之趨勢。基因型間乾物質之累積量以TA85-17×H95最大，其次為A661，最小者為ICAL210及TA85-17。相關分析顯示地上部乾物累積重、根部乾物累積重、整株乾物累積重、地上部／根部比值及GS活性與株齡間呈極顯著正相關；而GDH(glutamate dehydrogenase)活性及蛋白質含量與株齡間呈現顯著負相關，即表示GDH活性及蛋白質含量隨著株齡增大而遞減。GS、GOGAT(glutamate synthase)和GDH之間及其與乾物累積重間無相關性存在。蛋白質含量與GDH活性間為顯著正相關；而蛋白質含量則與地上部乾物累積重、根部乾物累積重及整株乾物累積重間呈極顯著負相關。

關鍵字

玉米 ； 銨 ； 幼苗乾物累積重 ； 銨同化酵素 ； 相關

並列摘要

To evaluate the relationships between ammonium assimilation enzyme activity and dry weight accumulation in maize seedling, seedlings of 4 inbred lines and 4 hybrids were grown in sand culture and supplemented with 50 mM NH4(superscript +) concentration at different plant stages in green house. Seedling harvested at 18 days after supplemented ammonium-nitrogen (NH4(superscript +)-N) and determined for gross differences in dry mass and ammonium assimilation enzyme activities. The results revealed that the plant stage, genotype and genotype x plant stage interaction were significantly different for all traits in maize seedling. The GS (glutamine synthetase) activity, shoot dry weight accumulation, root dry weight accumulation, total dry weight accumulation, and the ratio of shoot/root were up follow by plant stages increasing. The TA85-17×H95 has largest total dry weight accumulation, next by the A661 and the ICAL210 and TA85-17 has smaller total dry weight accumulation. The correlation analysis indicated that there has positive correlation between plant stages and GS activity, shoot dry weight accumulation, root dry weight accumulation, total dry weight accumulation, and the ratio of shoot/root in maize seedling. But there has negative correlation between plant stages and GDH (glutamate dehydrogenase) activity, protein content. Besides, the enzymatic activities of GS, GDH, and GOGAT (glutamate Synthase) and the increase of biomass in different genotypes of maize did not show any correlation relationship. Also there were non-correlation between ammonium assimilation enzymes activities and dry weight accumulation in maize seedling. The protein content and the GDH activity had positive correlation. But the protein content between shoot dry weight accumulation, root dry weight accumulation, total dry weight accumulation, and the ratio of shoot/root had negative correlation.

並列關鍵字

Maize ； Ammonium NH4(superscript +) ； Seedling dry weight accumulation ； Ammonium assimilation enzyme ； Correlation