In this study,we examine the changes of the hematological parameters of grey mullet(Mugil cephalus),milk fish(Chanos chanos),red snapper(Lutijanus argentimaculatus),andblack porgy(Acanthopagrus schlegeli)after anesthetization by using 2-phenoxyethanol.Theresults show that the 2-phenoxyethanol can significantly increase the values of hematocritof red snapper,hemoglobin of grey mullet,and osmolarity of black porgy.Furthermore,the specific sensitivity of the species to 2-phenoxyethanol also really exists,and differentspecies would demonstrate a few different physiological changes during anesthesia.