Studies on Lactic Acid Bacteria Fermented Seaweed Polysaccharide Hydrolysates Solution of Grateloupia filicina

乳酸菌發酵蜈蚣藻多醣水解液之研究

吳紹祺(Shao-Chi Wu) ； 洪英豪(Ying-Hao Hung) ； 潘崇良(Chorng-Liang Pan) ； 邱思魁(Tze-Kuei Chiou)

《臺灣水產學會刊》 37卷1期 (2010/03) Pp. 13-23

https://doi.org/10.29822/JFST.201003.0002

摘要

本研究探討將蜈蚣藻多醣萃取液以酵素水解後經乳酸菌發酵之製程。蜈蚣藻熱水萃取液(Gra)經500 units MA-103的洋菜酶和500 units的纖維素酶於40℃降解12小時後可得蜈蚣藻水解液(L-Gra)。以(A) 5% Lactobacillus (Lb.) rhamnosus BCRC14068、(B) 5%Enterococcus (Ent.) faecalis BCRC13076、與(C) 2.5% Lb. rhamnosus BCRC14068及2.5% Ent. faecalis BCRC13076於37oC分別發酵(1) 0.5%蜈蚣藻多醣液經agarases及cellulase (500 unit)水解所得水解液及(2)該蜈蚣藻水解液添加0.5% sucrose、1% beefextract、及0.5% yeast extract (CN)，共得六組乳酸發酵液。結果顯示隨著發酵時間的增加，pH值隨著下降。其中以L-Gra-CN-F(下標 C5)組Lb. rhamnosus BCRC14068 (2.5%)及Ent. faecalis BCRC13076 (2.5%)發酵外加碳源與氮源的蜈蚣藻水解液反應至9 hr時，發酵液之pH值即可降至4.6以下，得一安全性產品，可滴定酸度為0.74%，乳酸菌數9.39 log CFU/ml。

關鍵字

洋菜酶 ； 纖維素酶 ； 乳酸菌 ； 蜈蚣藻

並列摘要

The purpose of this study is to develop the enzyme-digested hot water polysaccharide extract of Grateloupia (G.) filicina fermented by lactic acid bacteria (LAB). Polysaccharide extracts of G. filicina were digested by 500 units of cellulase and 500 units of agarases produced from Pseudomonas vesicularis MA103. Six LAB fermentation solutions were obtained from media: (1) the hydrolysate of 0.5% aqueous solution of the polysaccharide extracts of G. filicina digested by 500 unit agarase and cellulase or (2) this G. filicina hydrolysate added 0.5% sucrose, 1% beef extract, and 0.5% yeast extract. Each of these two media was fermented in three LAB groups: group A: 5% Lactobacillus (Lb.) rhamnosus BCRC14068, group B: 5% Enterococcus (Ent.) faecalis BCRC13076, group C:2.5% Lb. rhamnosus BCRC14068 and 2.5% Ent. faecalis BCRC13076. The results of the pH values of the six LAB fermentation solutions showed a decrease as the fermentation time increased. The pH value of the LAB fermentation solution that the LAB group C cultured in the G. filicina hydrolysate with added carbon and nitrogen source (L-Gra- CN-F(subscript C5)) was below 4.6 with a fermentation time of 9 hr. The titratable acidity was 0.74% and lactic acid bacteria count was 9.39 log CFU/ml. The results could have potential for use in the future development of lactic fermentative G. filicina oligosaccharide hydrolysate health foods.

並列關鍵字

Agarases ； Cellulase ； Lactic acid bacteria ； Grateloupia filicina