Biological Activities of Monostroma nitidum Wines

青海菜酒之生理活性

吳紹祺(Shao-Chi Wu) ； 曾國政(Kuo-Chang Zen) ； 陳君如(Jiun-Ru Chen) ； 潘崇良(Chomg-Liang Pan)

《臺灣水產學會刊》 38卷4期 (2011/12) Pp. 301-316

https://doi.org/10.29822/JFST.201112.0003

摘要

本研究係以酵母菌發酵青海菜（綠藻）後產得的青海菜酒之抗氧化活性與抗致突變性的變化。青海菜酒在螯合亞鐵離子能力方面較海菜水萃液及水解液為弱，僅17.9-29.8%。清除α,α-diphenyl-β-picrylhydrazyl（DPPH）自由基能力則優於海菜水萃液及水解液，為65.3-69.1%。而Trolox當量的抗氧化能力（Trolox equivalent antioxidant capacity, TEAC）方面隨青海菜酒中乙醇含量的增加而提高，以添加20% sucrose並接種S6菌組發酵之青海菜酒有最佳的效果，達1.9 mM。青海菜酒對於4NQO所誘導Salmonella typhimurium TA98及TA100突變之抑制率分別為37-48%與52-63%；而對於Benzo[a]pyrene（B[a]P）所誘導TA98及TA100突變的抑制效果則分別呈現28-45%與37-55%之抑制率。青海菜酒所呈現抗氧化能力和抗致突變性與其所含多酚類相關。

關鍵字

抗氧化性 ； 抗致突變性 ； 青海菜酒 ； 螯合亞鐵離子能力 ； 清除DPPH自由基能力 ； Trolox當量的抗氧化能力（TEAC）

並列摘要

The subject of this research is to investigate the changes on antioxidative properties and antimutagenicity of the Monostroma (M.) nitidum wine, which made from M. nitidum (green alga) via fermentation with yeasts to produce green algal wine. As to the ability of chelating on ferrous ions of M. nitidum wines, the tested results indicated that M. nitidum wines were inferior to M. nitidum extract and its hydrolysate solution. The α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging effect of M. nitidum wines ranged from 65.3% to 69.1%, which were better than M. nitidum extract and hydrolysate solution. The Trolox equivalent antioxidant capacity (TEAC) of M. nitidum wines increased with an increase in their ethanol content, and the M. nitidum wines with 20% sucrose added and fermented by the S6 yeast group obtained the highest TEAC (1.9 mM). The inhibitions of M. nitidum wines against the mutagenicity induced by 4-NQO were evaluated by Salmonella typhimurium TA98 and TA100, reaching 37-48% and 52-63% inhibition of mutagenesis, respectively. The inhibition effect of M. nitidum wines against the mutagenicity induced by indirect-acting mutagen Benzo[a]pyrene (B[a]P) with S9 mix were evaluated by Sal. typhimurium TA98 or TA100, reaching 28-45% and 37-55% inhibition of mutagenesis, respectively. The antioxidative abilities and antimutagenicity activities values were correlated with the soluble total polyphenols content in M. nitidum wines.

並列關鍵字

Antioxidative properties ； Antimutagenicity ； Monostroma nitidum ； Wine