Effects of banana (Musa acuminata) fruit's peel pectin on the immune responses and disease resistance of the giant freshwater prawn, Macrobrachium rosenbergii

香蕉皮果膠對淡水長臂大蝦免疫反應及抗病能力之效果

蘇芳遙(Kamonwan Suphawinyoo) ； 張欽泉(Chin-Chyuan Chang) ； 鄭文騰(Winton Cheng)

《臺灣水產學會刊》 44卷2期 (2017/06) Pp. 75-87

https://doi.org/10.29822/JFST.201706_44(2).0001

摘要

The pectins isolated from fruit's peel of banana, Musa acuminata, was used to evaluate the potential immunostimulation, and the influence on disease resistance in giant freshwater prawn, Macrobrachium rosenbergii through injection. Prawn received banana peel water-soluble pectins (BWP) via injection at 10, 20 and 40 μg/(g prawn) significantly increased total haemocyte count (THC), hyaline cell (HC), granular cell (GC), phenoloxidase (PO) activity, lysozyme activity, and phagocytic activity and clearance efficiency against Lactococcus garvieae after 1, 3 or 7 days. For susceptibility test, M. rosenbergii was injected with BWP at 10, 20 and 40 μg/(g prawn) after 3 days then challenged with Lactococcus garvieae. The mortality of M. rosenbergii injected with BWP at 20 and 40 μg/(g prawn) were significantly lower than those injected with BWP at 10 μg/(g prawn) and saline control after challenge with L. garvieae for 48~144 hours, and had decreased by 30% and 40%, respectively, compared to the challenged control after 144 hours. It was concluded that the BWP can be used as an immunostimulant for prawn through injection to enhance immunity, and resistance against L. garvieae.

關鍵字

Macrobrachuim rosenbergii ； immunity ； pectins ； Musa acuminata ； immunostimulant

並列摘要

自香蕉皮分離之果膠經由注射方式評估其對淡水長臂大蝦可能的免疫刺激及抗病能力影響。蝦子注射10，20 and 40μg／（g prawn）香蕉皮水溶性果膠後的1、3或7天顯著提升總血球數、透明血球、半顆粒血球、顆粒血球、酚氧化酵素活性、溶菌酵素活性及乳酸鏈球菌吞噬與清除作用。在疾病感受性試驗中，淡水長臂大蝦注射香蕉皮水溶性果膠10、20和40μg／（g prawn）3天後再以乳酸鏈球菌進行攻擊。淡水長臂大蝦注射20和40μg／（g prawn）香蕉皮水溶性果膠組別於攻擊後的48～144小時之死亡率明顯比控制組低，且在攻擊後144小時分別顯著低於攻擊控制組30%與40%。因此，香蕉皮水溶性果膠可作為蝦類免疫刺激物，經由注射方式強化淡水長臂大蝦免疫及抗乳酸鏈球菌的能力。

並列關鍵字

淡水長臂大蝦 ； 免疫力 ； 果膠 ； 香蕉 ； 免疫刺激物