- 期刊
植物生長調節劑對於紫田薯組織培養生産花青素之影響
Effects of Plant Growth Regulators on Anthocyanin Production in Tissue Culture of Dioscorea alata L. var. purpurea M. Pouch
摘要
以紫田薯(Dioscorea alata L. var. purpurea M. Pouch)之薯塊、莖、節、葉培植體置於MS培養基中進行組織培養，探討不同濃度組合之植物生長調節劑對於紫紅色癒傷組織細胞誘發的影響。結果發現低濃度kinetin與高濃度2,4-D組合之效果較好，其中以10μM 2,4-D與0.1μ M kinetin濃度與組合效果最好。NAA及BAP各種濃度組合對於紫田薯紫紅色癒傷組織之誘發並無明顯的影響。而各種培植體中，以薯塊或節之紫紅色癒傷組織的産生率較高。將各培植體形成之紫紅色癒傷組織置於MS液體培養基中進行繼代培養，其細胞量及花青素之産生量均會增加，但若置於MS半固體培養基中進行繼代培養，則會有褐化現象産生。
並列摘要
Effect of plant growth regulators on initiation of purple calli from tuber, stem, node, and leaf explants of Dioscorea alata L. var. purpurea M. Pouch cultured on MS (Murashige and Skoog, 1962) media were studied. Adding 2,4-D and kinetin to the MS media promoted more purple calli than that of NAA and BAP. The best results were observed among treatments containing high concentrations of 2,4-D and low concentrations of kinetin. Supplements of 10μ M 2,4-D and 0.1 μ 58 kinetin to the MS medium induced more purple calli than those of other combinations. Tuber and node explants initiated more purple calli than stem or leaf explants. Purple calli subcultured on the liquid medium enhanced the production of purple cells. However, purple calli subcultured on semi-solid medium resulted in browning.
延伸閱讀
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