柳杉葉部精油及其成分之抗細菌活性評估

Evaluation of the Antibacterial Activities of Leaf Essential Oil and Its Constituents from Cryptomeria Japonica D. Don

古喬云(Chiao-Yun Ku) ； 鄭森松(Sen-Sung Cheng) ； 吳季玲(Chi-Lin Wu) ； 李文馨(Wen-Hsin Li) ； 張上鎮(Shang-Tzen Chang) ； 張惠婷(Hui-Ting Chang)

《中華林學季刊》 41卷2期 (2008/06) Pp. 237-247

https://doi.org/10.30064/QJCF.200806.0007

摘要

本研究目的為評估柳杉(Cryptomeria japonica D. Don)葉部精油的抗細菌活性，同時由精油中分離與鑑定具抗細菌活性的成分。試驗結果顯示柳杉葉部精油對於金黃色葡萄球菌(Staphylococcus aureus)與糞腸球菌(Enterococcus faecalis)的最低抑制濃度分別為2mg/mL與1mg/mL。進一步由活性分離部中分離得到ent-Kaur-16-ene、γ-Eudesmol、β-Eudesmol、β-Elemol、α-Cadinol與cis-Sabinene hydrate，其中，cis-Sabinene hydrate為首次自柳杉中分離與鑑定得到的化合物。由精油成分的抗細菌活性評估，得知β-Elemol、α-Cadinol與ent-Kaur-16-ene的抗細菌活性較佳，對於抗藥性金黃色葡萄球菌(Methicillin-resistant Staphylococcus aureus, MRSA)的最低抑制濃度均未超過0.5mg/mL。綜合上述結果可知柳杉葉部精油具有良好的抗細菌活性。

關鍵字

抗細菌活性 ； α-Cadinol ； 柳杉 ； β-Elemol ； ent-Kaur-16-ene ； cis-Sabinene hydrate

並列摘要

The objective of this paper was to evaluate the antibacterial activity of leaf essential oil from Cryptomeria japonica D. Don. Minimum inhibitory concentration (MIC) values of leaf oil were 2mg/mL against Staphylococcus aureus and 1mg/mL against Enterococcus faecalis respectively. Six terpenoids including cis-sabinene hydrate, γ-eudesmol, β-eudesmol, β-elemol, α-cadinol and ent-kaur-16-ene were isolated and identified from active fractions of leaf oil. Among these compounds, cis-sabinene hydrate was isolated for the first time from this plant. Results from antibacterial assay revealed that β-elemol, α-cadinol and ent-kaur-16-ene exhibited inhibitory effects against bacteria. MIC values of these three constituents were all less than 0.5mg/mL against methicillin-resistant Staphylococcus aureus (MRSA). Results obtained demonstrated that leaf oil of C. japonica possesses the commendable antibacterial activity.

並列關鍵字

Antibacterial activity ； α-Cadinol ； Cryptomeria japonica ； β-Elemol ； ent-Kaur-16-ene ； cis-Sabinene hydrate