細胞分裂素促進黛粉葉雄花序培植體體胚發生與植株再生

Cytokinins Stimulate Somatic Embryogenesis and Plant Regeneration from Male Inflorescence of Dieffenbachia 'Tiki'

沈榮壽(Rong-Show Shen) ； 李哖(Nean Lee)

《台灣農學會報》 10卷5期 (2009/10) Pp. 380-388

https://doi.org/10.6730/JAAT.200910_10(5).0003

摘要

This study aimed to explore the effect of cytokinins on increasing the somatic embryogenesis and subsequent plant regeneration of Dieffenbachia 'Tiki'. Male inflorescence explants isolated from spadix were cultured on a half-strength Murashige and Skoog (MS) medium, consisting of 2% sucrose, 1% glucose, 0.18% gelrite and 4 mg/L 2, 4 dichlorophenoxy acetic acid (2,4-D) for the basal medium, then add 0.5 or 1.0 mg/L N6-furfuryl adenine (Kinetin), 6-benzylaminopurin (BA) or Thidiazuron (TDZ). The percentage of somatic embryogenesis and number of mature embryoids increased with increasing concentration of cytokinins. The competency for inducing somatic embryogenesis is TDZ＞BA＞Kinetin in that order. Basal medium supplemented with 0.5 or 1.0 mg/L TDZ and 4 mg/L 2,4-D resulted in 100% somatic embryogenesis, and obtain an average of 35.2 somatic embryos per responding explant. This was the first report showing that some emblings developed from few mature embryoids in Dieffenbachia 'Tiki'.

關鍵字

黛粉葉 ； 雄花序 ； 細胞分裂素 ； 體胚發生 ； 植株再生

並列摘要

This study aimed to explore the effect of cytokinins on increasing the somatic embryogenesis and subsequent plant regeneration of Dieffenbachia 'Tiki'. Male inflorescence explants isolated from spadix were cultured on a half-strength Murashige and Skoog (MS) medium, consisting of 2% sucrose, 1% glucose, 0.18% gelrite and 4 mg/L 2, 4 dichlorophenoxy acetic acid (2,4-D) for the basal medium, then add 0.5 or 1.0 mg/L N6-furfuryl adenine (Kinetin), 6-benzylaminopurin (BA) or Thidiazuron (TDZ). The percentage of somatic embryogenesis and number of mature embryoids increased with increasing concentration of cytokinins. The competency for inducing somatic embryogenesis is TDZ＞BA＞Kinetin in that order. Basal medium supplemented with 0.5 or 1.0 mg/L TDZ and 4 mg/L 2,4-D resulted in 100% somatic embryogenesis, and obtain an average of 35.2 somatic embryos per responding explant. This was the first report showing that some emblings developed from few mature embryoids in Dieffenbachia 'Tiki'.

並列關鍵字

Dieffenbachia ； Male inflorescence ； Cytokinins ； Somatic embryogenesis ； Plant regeneration