落花生栽培種未熟胚誘導體胚及植株再生之研究

Somatic Embryogenesis and Plant Regeneration from Immature Embryos of Peanut (Arachis hypogaea)

胡澤寬(Tzer-Kaun Hu) ； 徐敏洲(Min-Chou Hsu)

《農林學報》 51卷3期 (2002/09) Pp. 43-55

https://doi.org/10.30089/JAF.200209.0004

摘要

本試驗以落花生栽培種之未熟胚為材料，探討2,4-dichlorophenoxyacetic acid(2,4-D)誘導體胚，及naphthaleneacetic acid(NAA)與抗壞血酸促進發根的效果，期望建立經由體胚形成再生植株之方法。將臺南11號之上胚芽與子葉基部組織培養於添加0.5, 1, 2, 4, 8 及16 mg/l 2,4-D的MS培養基中45天，結果顯示上胚芽之體胚誘導率及體胚形成數均高於子葉基部培植體。就2,4-D濃度而言，上胚芽培養於添加4 mg/l 2,4-D培養基有最高的體胚誘導率(57.39%)，且大多為正常之體胚；8mg/l以上的2,4-D則會提高培植體褐化率而降低體胚誘導率，且所形成的體胚大多為不正常的融合胚。又以8個品種（系）之未熟上胚芽培養於MS+4 mg/l 2,4-D培養基得知，體胚誘導率在品種（系）間有顯著的差異。將體胚移入添加50 mg/l抗壞血酸及0.5mg/l NAA之培養基中，可獲致根系旺盛的再生幼株，該等再生植株經健化後，移植至土壤中均生長健旺及可正常開花。

關鍵字

落花生 ； 未熟胚 ； 體胚形成 ； 2,4-D ； NAA ； 抗壞血酸

並列摘要

In this study, the peanut (Arachis hypogaea L.) cultivars were used as materials to investigate the effect of 2,4-dichlorophenoxyacetic acid(2,4-D) on somatic embryogenesis and of naphthaleneacetic acid(NAA) and ascorbic acid on rooting, then to find a efficient protocols for regeneration of plantlet via somatic embryogenesis.
As immature epicotyle and the half proximal cotyledon of TN 11 were cultured on MS basal medium supplemented with 2,4-U at 0.5, 1, 2, 4, 8 and 16 mg/l, the results indicate that the induced rates of somatic embryo from epicotyle was better than from the half proximal cotyledon. Of all media with 2,4-D used, 4mg/l 2,4-D had the highest rate (57.93%) at embryogenesis and produced a more number of normal somatic embryo. As 2,4-D level in induction medium increased to 8 mg/l or more, the percentage of embryogenesis decreased and was associated with browning of explants, and most of somatic embryo morphology was abnormal. The genotypic effect on embryogenesis was significant. Somatic embryos germinated on the medium with 50 mg/l ascorbic acid and 0.5 mg/l NAA could develop to the well-rooted plantlets, and develop into healthy and fertile plants when planted in soil.

並列關鍵字

Arachis ； Immature embryo ； Somatic embryogenesis ； 2,4-D ； NAA ； Ascorbic acid