The effects of molecular weight fractionation using hollow fiber membrane on emulsifying properties of Monostroma nitidum mucilage were investigated. By gel permeation chromatography, it was observed that the low molecular weight fraction of protein of Monostroma nitidum mucilage was lower than 10 kDa and was easily adsorbed onto the oil/water intrerfaces after emulsification. The fractionated molecular weight larger than 10 kDa showed higher emulsifying activity and emulsifying activity index. Increase of fractionation time increased the amounts of polysaccharides and protein adsorbed on the oil/water interfaces, and also enhanced the emulsifying activity and emulsion stability from 48.3 and 47.8% to 53.4 and 52.3%, respectively.
The effects of molecular weight fractionation using hollow fiber membrane on emulsifying properties of Monostroma nitidum mucilage were investigated. By gel permeation chromatography, it was observed that the low molecular weight fraction of protein of Monostroma nitidum mucilage was lower than 10 kDa and was easily adsorbed onto the oil/water intrerfaces after emulsification. The fractionated molecular weight larger than 10 kDa showed higher emulsifying activity and emulsifying activity index. Increase of fractionation time increased the amounts of polysaccharides and protein adsorbed on the oil/water interfaces, and also enhanced the emulsifying activity and emulsion stability from 48.3 and 47.8% to 53.4 and 52.3%, respectively.