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砂床培育帶菌根杉木苗之研究-優良菌種之篩選與繁殖(一)

Cultivation of China Fir (Cunninghamia lanceolata) Mycorrhizal Seedlings in Sandy Nursery-Select and Propagate the Effective Mycorrhizal Fungi. (Ⅰ)

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摘要


本報告對杉木共生之優良菌根菌種進行篩選與繁殖研究。供試土樣採自本所蓮華池分所杉木林根域及苗圃地,研究結果顯示蓮華池地區自杉木根域分離出之VA菌根菌種計有Suctellospora nigra, Gigaspora gigantea, Glomus mosseae. Gl. macrocarpum, Gl. tortusosum, Acaulospora laevis, Sclerocystis sinuosa及其他四種尚未鑑定之Glomus spp。和一種Acaulospora spp。上述十二種菌根菌經純培養繁殖,僅A. laevis和Gl. mossae可獲得大量孢子。盆栽試驗顯示此二種菌種均能有效地促進杉木苗之生長。而另外選自其他地區用以比較菌種有效性之A. laevis及Gi. margarita則效應較低或幾不具有效性。

關鍵字

VA菌根 杉木 砂床

並列摘要


The purposes of this study were: (1) to survey the VA mycorrhizal fungi (VAMF) occurring in China Fir (Cunninghamia lanceolata) stands; and (2). to select and propagate the most effective mycorrhizal fungi which could form mycorrhizal association with China Fir. Soil samples collected from the plantation and/or nurseries of China fir of Lien-Hua-Chin Branch Station, Taiwan Forestry Research Institute, were processed by wet sieving and decanting methods. Seven VAMF, namely, Scutellospora nigra, Gigaspora gigantea, Glomus mosseae, Gl. macrocarpum, Gl. tortusosum, Acaulospora laevis, Sclerocystis sinuosa along with 4 Glomus spp. and 1 Acaulospora spp. were noticed. These mycorrhizal fungi species were then propagated by sand culture in greenhouse. The results showed that only Acaulospora laevis and Glomus mosseae could produce mass spores from this culture. Potting growth experiment were conducted to compare the effectiveness of these two fungi in improving the performance of China Fir seedlings. Two other mycorrhizal fungi, Gi. margarita and A. laevis, selected from Ro-Ton and Young-Ming Mountain, respectively, were also included in this experiment. The results indicated that Gl. mosseae and A. laevis, both from Lien-Hua-Chih, were most effective; whereas A. laevis from Young-Ming mountain and Gi. margarita from Ro-Ton were less effective in improving the performance of China Fir.

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