Healing of Central Tendon Defect: A Histomorphometrical Study

This investigation was designed to do a quantitative historlgical study on the healing processes of the central defects in the canine quadriceps tendons. Specific central tendon defects, including upper half, lower half, whole layer with suture, and whole layer without suture, were created surgically in the quadriceps tendons of thirty-tow legs of 10-15Kg mongrel dogs. Histgological studies were performed on the quadriceps tendons after these animals were sacrificed sequentially at the 2 wk, 5wk, 8wk, and 12 wk post-operatively. Genias program (JoyceLoevi ltd, England) was used to analyze the dense fiber areas, matrix areas, and the nuclear areas of all the tissue sections, including two control and two sham control. The percentage of dense fiber area increased greatly, especially during the first five weeks, and reached 85-92% in all the groups at 12th week after operations. Remodeling of the fibers was also noted. However, the arrangement and polarization of the collagen fibers was still different from the control at the twelve week post-operatively. The areas of both the matrix and nucleus of all the specimens were decreasing to less than 14% and 3% respectively at 12th wk post-operatively. The results of our histomorphometrical studies were compatible with the biochemical analysis of healing tendons from other report. This study showed that the healing processes of central tendon defects were dependent on the lesion types and with suture repair or not. However, the effects of synovial fluid on tendon healing, and the healing process of various tendon defects need more studies.

並列關鍵字

Tendon defect ； Healing ； Histomorphometry

國際替代計量

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