培養基成分對四季蘭‘金荷’根莖生育及分化之影響

Effects of Medium Composition on Growth and Differentiation of Rhizomes of Cymbidium ensifolium

陳季呈(Chi-Cheng Chen)

《花蓮區農業改良場研究彙報》 29期 (2011/12) Pp. 13-24

https://doi.org/10.6957/RBHDAIS.201112.0013

摘要

四季蘭‘金荷’根莖於MS基礎培養基中添加40 g/l蔗糖處理下生育情形良好，其外觀較為粗壯，且繁殖倍率較高，而香蕉泥及蘋果泥處理雖會造成培植體褐化，但新生之根莖較為粗壯，有利於日後芽體之誘導與生成。植物生長調節劑NAA可促進根莖生育，BA及TDZ則可促進根莖分化芽體，其中以BA 0.5-1 mg/l或TDZ 0.1 mg/l搭配NAA 0.1 mg/l組合處理較為適宜，芽體外觀呈細長狀；當BA處理濃度達5 mg/l時培植體易發生褐化，而不利芽體形成，且其無褐化之芽球外觀與TDZ 0.5-1 mg/l所誘導之芽體均呈短圓形，無法正常生長，比較TDZ與BA處理之效果，TDZ誘導根莖分化芽體較BA顯著。

關鍵字

四季蘭 ； 組織培養 ； 植物生長調節劑 ； 器官分化

並列摘要

Seed-derived rhizomes of Cymbidium ensifolium 'Jin-he' were grown well in full MS medium and 40 g/l sucrose treatment. Their appearances were thicker, and the reproductive rates were higher than in other treatments of the salt-base strength and sucrose concentration. Although organic additives of banana or apple treatments caused rhizome browning, the rhizomes were thicker and viable for future induction and generation of shoots. Using plant growth regulator NAA to promote rhizome growth, BA and TDZ to promote the differentiation of rhizomes into shoots, it was found that BA 0.5-1 mg/l or TDZ 0.1 mg/l combined with NAA 0.1 mg/l produced differentiation, optimum into elongated shoots with no browning. When the concentration of BA was increased to 5 mg/l, the plantlets were browning more severely, and there was no shoot formation. The appearance of non-browning buds treated with 5 mg/l BA and TDZ 0.5-1 mg/l was short and round, and they did not grow normally when planted. Comparing TDZ with BA for promoting rhizome differentiation effects, TDZ-induced shoot formation were significantly better than with BA.

並列關鍵字

Cymbidium ensifolium ； tissue culture ； Plant growth regulator ； organogenesis