臨床真菌之分子鑑定與DNA晶片檢測技術

Using of Molecular Methods and DNA Microarray for the Detection and Identification of Clinical Fungal Infections

劉向寧(Shiang-Ning Leaw)

《科儀新知》 178期 (2010/10) Pp. 38-47

https://doi.org/10.29662/IT.201010.0005

摘要

侵入性真菌感染在近二十年來有逐漸增加的趨勢，目前此類感染症的死亡率大約是30-50%。傳統的鑑定方法需要不少人力與時間才能正確鑑定菌種。隨著抗真菌藥物的進展，提供了不同種別及抗藥性投藥的多種選擇，使得正確種別鑑定更形重要。所以研發出快速鑑定感染性真菌的方法將有利於迅速給藥，以期提高治療效果與降低死亡的機率。本文從真菌的分類及其所引起的疾病，進一步介紹各種分子鑑定方法，最後討論目前最受矚目的DNA晶片與其發展趨勢。

並列摘要

The frequency of invasive fungal infections has increased in the past 20 years. The mortality rate for invasive fungal infection is 30-50%. The conventional methods for fungal identification required are labor-intensive and time consuming. The development of species-specific antifungal drugs has provided more treatment selections, thus identification of the fungal species has becoming more important. Rapid identification of fungal infections will provide appropriate treatment and thus reduce morbidity and mortality. This article will introduce the fungal classification and diseases caused by the infections. Then further describes the commonly used molecular identification methods. Finally DNA array technology is discussed.