正常及不正常眼結膜之捺壓細胞學

Impression Cytology in Normal and Diseased Conjunctiva

梁如蘭(Lu-Lan Liang) ； 陳昌文(Chang-Wen Chen) ； 盧雪玉(Hsuei-Yuh Lu)

《中華民國眼科醫學會會刊》 26卷1期 (1987/05) Pp. 315-322

https://doi.org/10.30048/ACTASOS.198705.0056

摘要

榮總自1986年3月至1986年9月收集100名病患做眼結膜捺壓細胞學之研完。其中包括20名正常無眼疾者，26名結膜炎患者，11名因眼睛乾燥而感不適但不符乾眼症診斷標凖者，38名乾眼症患者，5名其他病患者。作者將所做出的眼結膜捺壓細胞學依麟狀化生(squamous metaplasia)之觀點分為六期，即0期至5期。正常者之球結膜應屬1期至2期範圍之間，瞼結膜應屬0期至l期範圍之間。結膜炎患者，杯狀細胞略為增生，乾眼症患者，若眼疾愈嚴重，則眼結膜捺壓細胞學之期數愈高。我們認為眼結膜捺壓細胞學對眼表面疾病尤其是亁眼症，是一種簡易且有效的診斷及評估療效的工具。

並列摘要

Using conjunctival impression cytology technique we studied 20 normal subjects and 80 patients with various ocular surface disorders since March 1986 to Sept. 1986. Six different cytological stages were defined on the basis of changes in goblet cell density, nucleus and cytoplasm, encompassing four major steps (1) loss of goblet cells, (2) abnormal epithelial differentiation, (3) nucleus degeneration including chromatin distortion, pyknosis and lysis of nucleus, (4) keratinization.
Compared to other staining methods, modified PAS-Gill's hematoxylin stain could exhibit more remarkable contrast between goblet cell and nongoblet epithelial cell and made interpretation easier. Goblet cell hyperplasia can be noted in various conjunctivitis. The staging number of cytology had positive correlation with the severity of dry eyes. The therapeutic efficacy of topical vitamin A treatment was supported by impression cytology which showed marked improvement of squamous metaplasia in 6 of the 12 treated patients during this half year. The staging system allowed us to correlate pathological changes and clinical finding and to investigate the efficacy of treatment in various ocular surface disorders.

並列關鍵字

impression cytology ； goblet cells ； keratinization ； squamous metaplasia