Cellular Senescence and DNA Synthesis in Human Cataractous Lens Epithelial Cells

人類白內障水晶體上皮細胞之細胞老化及DNA合成

葉則祥(Tse-Shiang Yeh) ； 蔡長祐(Chang-Youh Tsai) ； 張武修(Wushou P. Chang) ； 黃嵩立(Song-Lih Huang) ； 王世叡(Soo-Ray Wang) ； 吳志翹(Chih-Chiau Wu) ； 張由美(Yu-Mei Chung) ； 李鳳利(Feng-Lih Lee) ； 李淑美(Shui-Mei Lee) ； 劉榮宏(Jorn-Hon Liu)

《中華民國眼科醫學會雜誌》 32卷2期 (1993/06) Pp. 146-152

https://doi.org/10.30048/ACTASOS.199306.0002

摘要

在生命體整個生命過程中，水晶體上皮細胞保有繼續增殖及分化成新的水晶體纖維的能力。此外，幾乎所有水晶體的代謝，合成及運輸過程都和其上皮細胞有關。因此，這些上皮細胞的功能障礙常於探討白內障的致病機轉時被提及。
在從前的實驗中我們發現，隨著白內障程度的增加，培養中的水晶體上皮細胞中，病態的細胞會明顯地增加。在此次的實驗中，我們進一步觀察於長期初培養中的上皮細胞的生長情形與檢定從30個有不同程度白內障的水晶體取得的上皮細胞在第一次次培養時的生長能力。
在長期初培養時，從較重程度白內障水晶體取得的上皮細胞自前囊向外生長的速度較緩慢，而細胞老化現象的出現較快速。逐漸地，細胞群中多呈含有絲狀細胞質構造的腫脹細胞且可見到多核巨細胞的出現。在第一次次培養時，隨著白內障程度的增加，水晶體上皮細胞對培養皿壁的黏貼能力及生長中利用培養基中3H-thymidine來合成DNA的能力都顯得較差，亦即有較差的增生能力。這顯示，在白內障形成中，水晶體上皮細胞內亦有逐漸老化的過程進行著。

並列摘要

Lens epithelia maintain the ability to proliferate and differentiate into new lens fibers throughout the whole life span. Besides, almost all the metabolic, synthetic and transport processes of the lens occur in them. Thus, malfunction in this part is often discussed in connection with the pathogenesis of cataract.
In our previous experiments, significantly increased pathologic cells have been observed among lens epithelia in culture as a function of increased degree of cataract. In the present investigation, we studied further the growth conditions of the epithelial cells in long-term primary culture and determined the growth capacity of those from 30 senile cataractous lenses with different degree of cataract after the first subculture.
In long-term primary culture, epithelial cells from lenses with more advanced cataractous degree showed a relatively slower cellular extension from the explant and presented signs of cell aging more rapidly with time. Gradually, bulky cells with filamentous structures predominated the cell population and multinucleated giant cells accumulated. In the first subculture, with the increase of cataractous degree, epithelial cells of the lens showed lower attachment ability to culture plates and decreased ability to incorporate 3H-thymidine into newly synthesized DNA, ie, decreased proliferative capacity. An intrinsic progressive aging of the lens epithelial cells is demonstrated in association with cataract formation.

並列關鍵字

human lens epithelial cells ； cataract ； growth capacity ； DNA synthesis ； reproductive death ； aging