以固定化Rhizopus oryzae BD5菌體進行大豆油轉酯化生產生質柴油之最適化研究

Rhizopus oryzae BD5篩自於受石油污染之土壤，其所生產的脂肪酶可將大豆油與醇類轉酯化成脂肪酸烷基酯與甘油，而此類的脂肪酸烷基酯即稱為生質柴油。本文乃先將此菌株在含有不織布纖維之Yeast Extract 0.5%、Pharmamedia 0.4%和Olive Oil 0.2%培養基中，於初始pH4.8、溫度30℃、轉速220rpm下培養72h後，再利用所得之固定化菌體進行轉酯化反應，並探討轉酯化操作變因（如：不同醇類、油醇比、固定化菌體含量及含水量等）對轉酯化效率之影響。對固定化之R. oryzae BD5菌體而言，其最適條件為使用正丁醇、醇／油比＝3、固定化菌體含量10wt.%、含水量10wt.%，以及反應時間為24h，其轉化率約可達65.2%。

關鍵字

生質柴油； Rhizopus oryzae ；細胞固定化；轉酯化；脂肪酶

並列摘要

Rhizopus oryzae BD5, which produced lipase to catalyze transesterification of soybean oil and alcohol to form fatty acid alkyl ester (so-called biodiesel) and glycol, was isolated from petroleum hydrocarbon-contaminated soils by selection method. The strain was cultured in optimal medium included nonwoven fibers using a composition with yeast extract 0.5% (w/w), pharmamedia 0.4% (w/w) and olive oil 0.2% (w/w) for intercellular lipase production. After 72 h fermentation, the R. oryzae BD5 immobilized cell was collected to transesterificate with various operating conditions. The optimal conditions for fatty acid alkyl esters production by transesterification with the immobilized cell were a 1-butanol/soybean oil molar ratio 3: 1, immobilized cell concentration 10 wt.% and minimum of 10 wt.% added water. The 65.2% conversion rate of fatty acid alkyl esters was obtained in 24 h at 30℃.

並列關鍵字

Biodiesel ； Rhizopus oryzae ； Cell Immobilization ； Transesterification ； Lipase

國際替代計量

以固定化Rhizopus oryzae BD5菌體進行大豆油轉酯化生產生質柴油之最適化研究

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