使用固定化的Candida Antarctica 脂肪分解酵素轉酯化大豆油以產生生質柴油

Transesterification of Soybean Oil to Biodiesel Using Immobilized Candida Antarctica Lipase

黃信智(Hsin-Chih Huang)

指導教授 ： 王榮基

大同大學/工程學院/化學工程學系所/碩士(2010年)

摘要

本研究使用價格低的商業上固定化candida antarctica脂肪分解酵素在培養振盪器上轉酯化大豆油與甲醇以產生生質柴油。由結果顯示在批示反應系統中的最佳操作條件為：5 wt%相對於油的分解酵素重量；油醇比為1比4；1 wt%相對於油的水重量；反應溫度40℃；震盪頻率200 rpm；反應時間72小時，所得到的最大產率為88%。在使用tert-butanol 沖洗反應過後的lipase，將使lipase維持一定的活性，重複反應6次後產率依然維持在58%。以玻璃製流體化床反應器於常溫下的最佳操作條件為：5 wt%相對於油的酵素重量；油醇比為1比3；反應物流速200 ml/min；反應時間72小時，所得到的最大產率為63%。由結果顯示，轉酯化反應在常溫下依然有不錯的產率，應可使用於工業化的大量生產。

關鍵字

生質柴油 ； 轉酯化反應 ； 大豆油 ； Candida Antarctica 脂肪分解酵素

並列摘要

The transesterification of soybean oil and methanol was investigated with a cheap, commercially immobilized enzyme from candida antarctica in an incubator shaker. The optimum conditions of the transesterification in batch system are as follows: 5 wt% lipase based on oil weight; oil/methanol molar ratio 1:4; 1 wt% water; reaction temperature at 40℃; vibration speed at 200 rpm; reaction time for 72 h, and the maximum methyl esters yield is 88%. The yield is 58% after being repeatedly used for 6 times when tert-butanol is adopted to wash lipase. The optimum conditions of the transesterification in a glass-made fluidized bed at room temperature are as follows: 5% lipase based on oil weight; oil/methanol molar ratio 1:3; flow rate at 200 ml/min; reaction time for 72 h, and the maximum methyl esters yield is 63%. The results show that the transesterification reaction can be processed in mass production at room temperature.

並列關鍵字

Biodiesel ； Transesterification ； Soybean Oil ； Candida Antarctica Lipase

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