松生擬層孔菌（Fomitopsis pinicola）成分分析及抗發炎之生物活性研究

Analysis of Chemical Components and Biological Activity of Fomitopsis pinicola

林家玉(Chia-Yu Lin)

《臺東區農業改良場研究彙報》 19輯 (2009/04) Pp. 53-70

https://doi.org/10.6959/RBTDAIS.200904.0053

摘要

開發高產且優質的藥用真菌，是本研究的目的。松生擬層孔菌，菌絲體乾重可達6.55 g/l的產量，多醣體產量為0.38 g/l，乙醇萃取物為17％（乙醇萃取物／菌絲體乾重）。在生物活性方面，在1000 μg/ml的濃度下，證明菌絲多醣體及乙醇萃取物都不具有生物毒性。在測定是否有抗發炎的能力方面，松生擬層孔菌萃取之多醣體，沒有明顯的藥效顯示；在乙醇萃取物方面，在100 μg/ml的濃度處理下，可以降低58%的human interferon gamma inducible protein-10（IP-10）蛋白質表現。

關鍵字

松生擬層孔菌 ； 多醣體 ； 抗發炎

並列摘要

The objective of this study was to develop high quality and yield medicinal fungi. Fomitopsis pinicola (Swartz. Fr.) Karst., were cultured for 35 days and the mycelia dry weight at the level of 6.55 g/l. The yield of polysaccharide of Fomitopsis pinicola was 0.38 g/l, and the ethanolic extraction was about 17% (ethanolic extraction/mycelia dry weight).The biological assay included polysaccharides, and ethanolic extract. The MTT test showed no toxicity to ECV (human vascular endothelial cell) cells up to a concentration of 1000 μg/ml. Interferon-γ inducible protein 10 (IP-10) induced by interferon-γ plays an important role in cell inflammation. By the expression of IP-10, the present result showed no effect for the polysaccharides of Fomitopsis pinicola. Among the ethanolic extract of Fomitopsis pinicola inhibited IP-10 protein release by 58% at concentration of 100 μg/ml.

並列關鍵字

Fomitopsis pinicola ； polysaccharides ； anti-inflammation